加工方法对毛豆中大豆异黄酮苷元含量的影响及其对糖尿病小鼠的降血糖降血脂活性研究  

Determination of Soy Isoflavone Aglycone Content of Edamame Prepared by Different Processing Methods and Its Hypoglycemic and Hypolipidemic Activity for Diabetic Mice

作　　者：刘璐璐[1] 王洲婷 丁传波[1] 李爽[1] 李伟[1] 刘文丛[1] 郑毅男[1] 

机构地区：[1]吉林农业大学中药材学院,长春130118

出　　处：《天然产物研究与开发》2014年第10期1659-1663,共5页Natural Product Research and Development

摘　　要：本文比较不同处理方式下毛豆中大豆异黄酮苷元的含量,继而对冻干条件下处理的毛豆样品进行降血糖降血脂活性的研究。采用高效液相色谱(HPLC)法测定通过热烫、煮沸、冷冻、冻干、烘干从毛豆中提取出的大豆异黄酮苷元的含量。HPLC测定条件如下:色谱柱:Hypersil C18(250 mm×4.6 mm,5μm),流动相:甲醇-0.3%磷酸溶液(48∶52),流速:0.7 mL/min,测定波长:260 nm,柱温:25℃,进样量:20μL。留一组健康小鼠作为空白,将造模成功的ICR小鼠随机分为3组,分别用生理盐水,消渴丸,冻干毛豆,对其连续灌胃4周,4周后处死,测定其血糖和血脂指标。结果显示,冻干条件下,大豆异黄酮苷元含量最多(115.45μg/g),并且冻干的毛豆能够显著改善糖尿病小鼠的血糖和血脂水平。This paper compares soy isotlavonc aglycone content of edamame prepared by different processing methods, and then study its hypnglycemic and hypolipidemie activity. Determination isoflavone aglycone content of edamame by high performance liquid chromatography （HPLC） methods in blanching,boiling,freezing,freeze-dried,drying conditions respectively. HPLC conditions ： Column： Hypersil Cn （250 mmx 4.6 ram,5 p,m）, mobile phase： methanol -0.3% phos- phoric acid solution （48： 52） ,flow rate ：0.7 mL/rain,detection wavelength：260 nm,column temperature ：25 ℃ ,the injection volume ：20 μL. Leaving a group of healthy mice were used as blank,successful modeling ICR mice were ran- domly divided into three groups, each with saline, xi^ke pills, freeze-dried edamame,its intragastrically four weeks, after four weeks, determination of blood glucose and lipid levels. The results showed that in freeze-dried condition, the most a- bundant isoflavone aglycone （ 115.45 μg/g）, and freeze-dried edamame can improve the blood sugar and lipid leveh sig- nificantly.

关 键 词：毛豆 大豆异黄酮 定量分析 血糖 血脂 

分 类 号：R965.1[医药卫生—药理学]