直接扩增技术在接触性检材DNA检验中的应用  

Application of direct amplification of DNA in different contact materials

作　　者：李长征[1] 刘海东[1] 崔文[2] 寻超群 孙贤学[1] 姜铭章[1] 

机构地区：[1]济宁市公安局刑侦支队,山东济宁272000 [2]济宁医学院法医学与检验医学学院,济宁272067

出　　处：《济宁医学院学报》2014年第5期347-349,共3页Journal of Jining Medical University

摘　　要：目的：将DNA直接扩增技术引入案件现场不同的接触性检材的检验中，构建有效的接触性DNA的生物检材的检验方法。方法采用剪取、擦拭等方法对手套、烟蒂、衣服、饮料瓶、吸管及残缺指纹等检材进行取样，采用DNA直接扩增技术获得短串联康复序列（short tandem repeat ，STR）分型。结果 DNA直接扩增技术在5种不同检材中检出的基因型均为单一个体来源，其对手套、烟蒂、衣服、饮料瓶和吸管、残缺指纹检材的成功率分别为90％、100％、70％、90％和60％，平均成功率在70％以上。分别对5种检材多处取点样品进行平行扩增，手套、烟蒂检材3个点位成功率均在90％以上；饮料瓶、吸管3个点位的成功率达80％～90％；衣服3个点位的成功率在60％～70％；残缺指纹3个点位的成功率在50％～60％。结论 DNA直接扩增技术对接触性DNA检材检验具有较高的有效应，其结果可靠，重复性强。Objective The effective protocal was constructed for DNA of different contact biological materials at the crime scene using direct amplification of DNA .Methods The samples were selected from contact materials such as gloves ,cigarette end ,clothes ,beverage bottle ,suction pipe and incomplete fingerprint by scissors and wi-ping .Then STR type was obtained using direct PCR .Results Genotype derived from five contact materials was the origin of a single individual by direct amplification of DNA .The successful rates were 90% ,100% ,70% ,90%and 60% in gloves ,cigarette end ,clothes ,beverage bottle ,suction pipe and incomplete fingerprint ,respectively . And the average successful rate was more than 70% .Parallel amplification was used for five contact materials by multidraw .The successful rate was more than 90% from 3 points in gloves and cigarette end ,80% ~90% in bev-erage bottle and suction pipe ,60% ~70% in clothes ,and 50% ~60% in incomplete fingerprint .Conclusion The direct amplification of DNA is efficient for DNA of contact materials .The results are reliable and good repeatabili-ty .

关 键 词：法医物证 接触性DNA 直接扩增 

分 类 号：DF795.2[医药卫生—法医学]