响应面法优化绿色木霉H06产孢培养条件  

Optimization of fermentation conditions for sporulation quantity by Trichoderma viride strain H06 using response surface methodology

作　　者：梁昌聪[1] 刘磊[1] 张建华[1] 郭立佳[1] 杨腊英[1] 王国芬[1] 黄俊生[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所/农业部热带作物有害生物综合治理重点实验室/海南省热带农业有害生物检测与控制重点实验室,海南海口571101

出　　处：《广东农业科学》2014年第19期80-85,共6页Guangdong Agricultural Sciences

基　　金：国家公益性行业(农业)科研专项(200903049)

摘　　要：在单因素试验的基础上,采用响应面法(response surface methodology,RSM)对绿色木霉菌H06菌株产孢培养条件进行了优化。首先利用Plackett-Burman试验设计筛选出影响产孢的3个主要因素:装液量、接种量和培养温度。在此基础上运用最陡爬坡路径法逼近最大响应值区域,最后利用响应面分析法确定主要因子之间的交互作用及最佳条件。结果显示,绿色木霉H06菌株的最佳发酵条件为:转速200 r/min,装液量74.70 mL/250mL,接种量5.83%,pH 7.5,温度29.50℃,培养时间72 h,菌龄48 h。H06菌株最大理论孢子含量为7.64×109个/mL。经3次平行试验验证,实际平均孢子含量与预测孢子含量相近,比之前的孢子含量提高133%。The fermentation conditions for sporulation quantity by Trichoderma viride strain H06 was optimized by response surface methodology（RSM） based on single factor tests. Firstly, three of the most significant influence factors were screened by the method of Plackett-Burman design as loading volume of liquid medium, inoculum volume and temperature.The path of steepest ascent was applied to approach the optimal region of the three significant factors. Lastly, the optimal concentration and correlations among three factors were identified by RSM. The optimal fermentation conditions were determined as followed： rotate 200 r/min, loading volume of liquid medium 74.70 mL/250 mL,inoculum volume 5.83%,pH7.5,temperature 29.50℃,time 72 h,bacterium age 48 h. Under these conditions,the maximum theoretic spore number was7.64×10^9mL^-1. After three parallel verifications,the maximum theoretic value was consistent with mean value of verification test and the spore number increased by 133% compared to that before optimization.

关 键 词：响应面法 绿色木霉 产孢 最适培养条件 

分 类 号：S482.292[农业科学—农药学]