响应曲面优化超临界CO_2萃取印楝素A工艺研究  

Optimization of supercritical CO_2 extraction of azadirachtin A by response surface methodology

作　　者：马李一[1] 王有琼[1] 张重权[1] 马金菊[1] 段琼芬[1] 

机构地区：[1]中国林业科学研究院资源昆虫研究所,云南昆明652224

出　　处：《广东农业科学》2014年第19期94-99,共6页Guangdong Agricultural Sciences

基　　金：国家"十二五"科技支撑计划项目(2012BAD21B0404)

摘　　要：运用响应曲面设计(Box-Behnken)优化超临界CO2萃取印楝素A提取工艺。在单因素试验基础上,以温度、压力、夹带剂用量为响应变量,印楝素A质量分数为响应值进行优化,模拟得到萃取印楝素A的二次多项回归方程模型:Y=0.3+0.067A+0.02B+0.043C+0.016AB-0.006AC+0.03BC-0.008A2+0.014B2-0.03C2,模型P<0.01,达到极显著水平;模型确定系数R2=0.9705,说明该模型与实际试验拟合较好。响应曲面分析法优选结果表明,超临界CO2萃取印楝素A的最佳工艺条件为萃取温度40℃、萃取压力28.65 MPa、萃取时间2 h,夹带剂用量2.79%,印楝素A实测结果0.40%与响应曲面拟合所得方程预测值0.42%吻合较好。采用响应曲面法建立的印楝素A提取工艺模型能很好地预测试验结果。Supercritical CO2 extraction technology on azadirachtin A from neem kernels was optimized by Box-Behnken center composite design. Taking extraction temperature, extraction pressure and entrainer dose as response variables, and azadirachin A（mass fraction） as response values, the extraction process was optimized by the method of response surface methodology and based on single-factor experiments and Box-Behnken central composite experimental design. The quadratic regression model for the extraction azadirachin A was as follows： Y=0.3＋0.067A＋0.02B＋0.043C＋0.016AB-0.006 AC ＋0.03BC-0.008A2＋0.014B2-0.03C2. The R2 was 0.9705, and P 0.01, reaching the level of very significance, demonstrating that the equation being well regression. The results showed that the optimal process conditions of supercritical CO2 extraction extracting azadirachtin A by response surface methodology were as follows： extraction temperature 40℃, extraction pressure 28.36 MPa and entrainer dose 2.79%; Under these conditions, the practical value of azadirachtin A was 0.40%, which agreed well with the predicted value of 0.42%. So extraction model of azadirachtin A established by Box-Behnken method precisely predicted the experiment results.

关 键 词：印楝素A 超临界 响应曲面法 萃取 夹带剂 

分 类 号：TQ453.3[化学工程—农药化工]