强鸡散对免疫抑制小鼠脾转录因子GATA-3和T-bet表达量的影响  

Effect of strong chicken powder on relative expression of GATA-3 and T-bet mRNA in mice spleen immunosuppressed by cyclophosphamide

作　　者：刘汉斌[1,2] 彭娇娇[1] 魏文康 翟少伦 熊惠军[1] 吕殿红 

机构地区：[1]华南农业大学兽医学院,广东广州510642 [2]广东省农科院动物卫生研究所,广东广州510640

出　　处：《广东农业科学》2014年第19期122-125,134,共5页Guangdong Agricultural Sciences

基　　金：广东省科技计划项目(2010B031000016);科技部星火计划项目(2012GA780002)

摘　　要：试验旨在通过定量检测小鼠转录因子T-bet、GATA-3 mRNA表达水平,探讨强鸡散对环磷酰胺致免疫抑制小鼠转录因子GATA-3和T-bet的调节作用。40只体重20 g左右的昆明纯种小鼠被随机分为4组:环磷酰胺组、中药组、环磷酰胺+中药组、对照组,采用实时荧光定量PCR方法,以β-actin基因作为内参基因,检测T-bet和GATA-3 mRNA的表达量。结果显示:强鸡散能改变小鼠脾脏转录因子GATA-3的相对表达量,对T-bet基因表达量没有明显影响。表明强鸡散具有调节环磷酰胺致免疫抑制小鼠脾脏转录因子GATA-3和T-bet表达量的作用,通过降低GATA-3的表达量,间接减少Th2细胞的生成,使Th1/Th2平衡向Th1方向转化。This study was designed to investigate the mRNA expressions of GATA-3 and T-bet of spleen in tested mice after immunosuppressed by cyclophosphamide, then intended to understand the regulation mRNA expression levels of GATA-3 and T-bet. 40 purebred ICR mice were divided into four groups randomly, including cyclophosphamide group,Chinese medicine group, cyclophosphamide and Chinese medicine group, the normal control group. Using fluorescent quantitative PCR, the GATA-3 mRNA and T-bet expression in spleen were investigated in the four groups, taking β-actin gene as a reference gene. The results suggest that strong chicken powder can regulate immune function through relieving immunosuppressive effects caused by cyclophosphamide and improving immune function inhibition.

关 键 词：强鸡散 荧光定量PCR GATA-3 T-BET 

分 类 号：S853.7[农业科学—临床兽医学]