鸡蛋清中金霉素残留的银胶表面增强Eu(III)的荧光检测  

Detection of Chlortetracycline Residues in Egg Albumen Using Silver Colloids Surface-Enhanced Fluorescence of Eu(Ⅲ)

作　　者：彭义杰[1] 刘木华[1] 赵进辉[1] 袁海超[1] 洪茜[1] 李耀[1] 

机构地区：[1]江西农业大学工学院/生物光电及应用重点实验室,江西南昌330045

出　　处：《激光与光电子学进展》2014年第10期211-216,共6页Laser & Optoelectronics Progress

基　　金：国家自然科学基金(31101295);江西省科技厅对外科技合作计划(20132BDH80005);江西省科技厅科技支撑项目(2012BBG70058);江西省教育厅科技计划(GJJ12244)

摘　　要：鸡蛋清中残留的金霉素（CTC）易进入人体内蓄积,从而对人体的健康产生损害。研究表明,银胶能作为表面基底来修饰金霉素与Eu（Ⅲ）的配合物,进而使配合物的荧光强度得到显著性的增强。本研究应用银胶表面增强Eu（Ⅲ）的荧光来检测鸡蛋清中金霉素的残留量,分别分析了不同胶体、银胶的加入量、Eu（Ⅲ）的加入量、反应时间等因素对荧光强度的影响,并确定了最佳的实验条件,在最佳条件下建立了以617 nm处的荧光特征峰强度（Y）与鸡蛋清中金霉素含量（X）之间的标定曲线。结果表明：鸡蛋清中金霉素浓度范围在1.5~29.5 mg/L时,鸡蛋清中金霉素含量与荧光强度之间呈现良好的线性关系,线性方程为Y=5.2265X＋24.033,决定系数R2为0.9043,实测值与预测值之间的R2=0.9182。可见用银胶表面增强Eu（Ⅲ）的荧光来快速检测鸡蛋清中金霉素的残留量是可行的。Chlortetracycline residues in egg albumen areaccumulated easily in humanbody, resulting in producing harm to human body. According to the research, silver colloids can be used as surface substrate to modify complexes of chlortetracycline and Eu（III）, which makes the fluorescence intensity of complexes enhanced significantly. Silver colloids surface-enhanced fluorescence of Eu（III） is applied to detect chlortetracycline residues in egg albumen. Different addition amount of colloid and Eu（III）, reaction time and other factors which have influence on fluorescence intensity are analyzed respectively in the research. The best experimental conditions are determined, and the calibration curve under the optimum condition is established between the fluorescence intensity of characteristic peak at 617 nm （Y） and chlortetracycline content （X）. The results show that a good linearity is obtained between the fluorescence intensity and chlortetracycline concentration in egg albumen in the range of 1.5-29.5 mg/L. The linear function is Y=5.2265＋24.033 X, and the determination coefficient is 0.9043. The determination coefficient between the measured values and predicted values is 0.9182. The results show that it is feasible to use silver colloids surface-enhanced fluorescence of Eu （III） to detect chlortetracycline residues in egg albumen rapidly.

关 键 词：光谱学 金霉素 荧光 银胶 Eu(Ⅲ) 

分 类 号：O657.3[理学—分析化学]