垂子买麻藤中抗菌化学成分的再研究  

Restudy on the Chemical Constituents with Antibacterial Activity from Gnetum pendulum

作　　者：陈丽君[1,2] 

机构地区：[1]浙江中医药大学药学院,浙江杭州310053 [2]浙江台州市立医院,浙江台州318000

出　　处：《药学进展》2014年第8期597-601,共5页Progress in Pharmaceutical Sciences

摘　　要：目的:进一步研究垂子买麻藤中抗菌化学成分。方法:利用正相硅胶柱层析、葡聚糖凝胶SephadexLH-20柱层析、半制备反相高效液相色谱等手段从垂子买麻藤茎杆的乙醇提取物中分离纯化得到9个单体化合物,采用电喷雾电离质谱和核磁共振氢谱等波谱分析方法对其进行结构鉴定,并以耐甲氧西林金葡菌(MRSA)为试验菌,考察其体外抑菌活性。结果:经波谱分析鉴定,分离所得9个单体化合物分别为原儿茶酸甲酯、买麻藤醇、白藜芦醇、原儿茶酸、香草醛、阿魏酸、isorhapontin、gnetifolinE和gnetuhaininA,其中原儿茶酸甲酯、买麻藤醇、原儿茶酸、香草醛、阿魏酸、gnetifolinE和gnetuhaininA为首次从该植物中分离得到;体外抑菌活性测试表明,原儿茶酸甲酯、白藜芦醇、原儿茶酸和香草醛对MRSA具有抑制活性,其最低抑菌浓度分别是2560、256、1280和2560mg·L-1,最小杀菌浓度分别是2560、1280、5120和2560mg·L-1,而其他单体化合物则无此活性。结论:垂子买麻藤中存在多种不同结构、不同活性的抗菌化学成分。To restudy the chemical constituents with antibacterial activity from Gnetum pendulum, Methods： Using normal phase silica gel column chromatography, Sephadex LH-20 gel column chromatography and semi-preparative RP-HPLC, nine compounds were isolated from the ethanol extract of Gnetum pendulum and their structures were identified by ESI-MS and 1H NMR. Using methicillin-resistant Staphylococcus aureus（MRSA） as test bacteria, the in vitro antibacterial activities of the isolated compounds were investigated,Results ： The nine isolated compounds were identified respectively as protoeatechuic acid methyl ester, gnetol, resveratrol, protoeatechuic acid, vanillin, ferulic acid, isorhapontin, gnetitblin E and gnetuhainin A by spectroscopic analysis, among which protoeatechuie acid methyl ester, gnetol, protoeatechuic acid, vanillin, ferulic acid, gnetitblin E and gnetuhainin A were isolated from this plant for the first time. In vitro tests of antibacterial activity showed that among them, protoeatechuic acid methyl ester, resveratrol, protoeatechuic acid and vanillin had potent antibacterial activities against MRSA, their MICs （mg.L-p） were 2 560. 256, 1 280 and 2 560 and MBCs （mg.L-~） were 2 560, 1 280, 5 120 and 2 560, respectively, but the others had no activity. Conclusion There are a variety of chemical constituents with different structures and antibacterial activities in Gnetum pendulum.

关 键 词：垂子买麻藤 乙醇提取物 化学成分 结构鉴定 耐甲氧西林金葡菌 体外抑菌活性 

分 类 号：R284.1[医药卫生—中药学]