浙江省46个粳稻新品种SSR指纹图谱构建及遗传多样性分析  

Fingerprint construction and analysis of genetic diversity with SSR markers for 46 japonica rice varieties in Zhejiang Province

作　　者：李小华[1,2] 叶胜海[2] 赵小燕[2,3] 姚坚[4] 刘继云[2] 陈蕾[2] 翟荣荣[2] 金庆生[2] 张小明[2] 

机构地区：[1]杭州师范大学生命与环境科学学院,浙江杭州310036 [2]浙江省农业科学院作物与核技术利用研究所,浙江杭州310021 [3]浙江师范大学化学与生命科学学院,浙江金华321004 [4]嘉兴市农业科学研究院,浙江嘉兴314016

出　　处：《浙江农业学报》2014年第5期1158-1163,共6页Acta Agriculturae Zhejiangensis

基　　金：浙江省科技厅项目(2012C12901-2);浙江省植物有害生物防控重点实验室-省部共建国家重点实验室培育基地;浙江省农业科学院提升工程项目

摘　　要：采用农业部推荐的12对水稻SSR引物（NY/T 1433—2007）,对2008年以来由浙江省主要育种单位育成的46个常规粳稻品种构建DNA指纹图谱,并分析其亲缘关系。结果显示,12对SSR引物共检测出84个等位基因,平均多态性频率（FP）值为0.192。46个品种的相似系数在0.735～1.000之间,平均相似系数为0.879,表明近年来浙江省粳稻品种亲缘较近,遗传背景较狭窄。该研究结果可为浙江省水稻新品种选育、鉴别和保护提供参考。In order to provide references for rice breeding in Zhejiang Province,12 pairs of rice SSR primers recommended by Ministry of Agriculture（NY /T 1433—2007） were adopted in the present research to construct DNA fingerprints for 46 japonica rice varieties bred in Zhejiang Province since 2008,as well as to analyze their genetic relationship. It was shown that 84 alleles were detected by 12 pairs of SSR markers,and the average frequency of polymorphism（FP） was 0. 192. The similarity coefficient of 46 cultivars was between 0. 735- 1. 000 with an average of0. 879,which indicated a close affinity among the tested cultivars and a narrow breeding background in Zhejiang Province.

关 键 词：常规粳稻 指纹图谱 SSR标记 遗传多样性 

分 类 号：S511[农业科学—作物学]