三十份菊芋资源亲缘关系的SRAP分析  

Analysis of genetic relationship of 30 Jerusalem artichoke germplasm resources by SRAP markers

作　　者：马胜超[1] 韩睿[1] 任鹏鸿 杨世鹏[1] 李莉[1] 

机构地区：[1]青海省农林科学院菊芋研发中心,青海省蔬菜遗传与生理重点实验室,青海西宁810016

出　　处：《浙江农业学报》2014年第5期1212-1217,共6页Acta Agriculturae Zhejiangensis

基　　金：国家大宗蔬菜产业技术体系西宁综合试验站(CARS-25-G-49)

摘　　要：利用SRAP分子标记技术对30份菊芋资源进行了基因组多态性分析。用22对扩增稳定且清晰的具有多态性DNA条带的引物组合,进行菊芋的SRAP-PCR扩增。共扩增出160条条带,其中多态性条带114条,多态性比率为71%,表明菊芋资源在DNA水平上存在广泛差异。利用NTSYS软件统计分析出资源间遗传距离在0.04～0.42之间。应用UPGMA得出聚类结果,将30份菊芋资源划分为2大类群,第2大类群分为6个亚类群。聚类结果与传统的形态学分类略有不同,可结合两种方法为菊芋资源亲缘关系的研究提供理论基础。Genetic diversity was analyzed in 30 accessions of Jerusalem artichoke using SRAP markers. 22 primer combinations were used for SRAP-PCR amplification of Jerusalem artichoke. 160 DNA fragments were amplified from30 samples and 114 fragments were polymorphic（percentage of polymorphic bands was 71%）,indicating that there were wide differences at DNA level among Jerusalem artichoke resource. The genetic distance indexes of 30 cultivars based on the SRAP data varied from 0. 04 to 0. 42. It suggested that the genetic diversity among resources of Jerusalem artichoke was high. Cluster analysis with UPGMA method showed that the 30 resources tested in this study could be divided into two groups. The second group could be divided into six sub groups. The clustering results were slightly different with traditional morphological classification. The above two methods could be combined to provide a theoretical basis for the study on the Jerusalem artichoke resource relationship.

关 键 词：菊芋 SRAP 亲缘关系 聚类分析 

分 类 号：S792.153[农业科学—林木遗传育种]