丹七片干预心肌缺血的血浆蛋白质组学研究  

Effect of Danqi Tablet on Plasma Proteome in Swines with Myocardial Ischemia

作　　者：郭淑贞[1] 啜文静[1] 王勇[1] 冯玄超[1] 廉洪建 郑磊[1] 王伟[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《中西医结合心脑血管病杂志》2014年第11期1355-1357,共3页Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease

基　　金：973计划课题(No.20D3CB517105);十二五支撑计划(No.2012BAI29B07);北京市自然科学基金(No.7112077)

摘　　要：目的探讨丹七片干预心肌缺血的作用机制。方法缩窄环法制备小型猪心肌缺血模型,给予丹七片每次9g/(只·天)临床等效剂量,4周。双向凝胶电泳-质谱联用法分析血浆蛋白表达谱变化。结果与模型组比较,丹七片下调蛋白点9个(丰度比值≤0.5)、上调蛋白点11个(比值≥1.5)。鉴定出的上调蛋白为载脂蛋白A1和载脂蛋白E。结论通过上调载脂蛋白A1和载脂蛋白E抑制缺血心肌过度的炎症反应,可能是丹七片干预心肌缺血的主要机制之一。Objective To explore the effect of Danqi table t(DQT) on plasma proteome in sw ines w ith myocardial ischem ia. Me thods Myocardial ischem ia was induced by a constrictoron the left coronary artery.After treated by DQT w ith a dose of 9 g per day for4 weeks ,two dimension e lectrophoresis andmass spectrome terwas used to analyze the changes ofplasma proteome .Re-sults The abundances of 9 prote in spots were decreased while 11 prote in spots increased in animals treated by DQT.Apolipopro-te in A1 and apolipoprote in E were identified as increased prote ins .Conclusion DQTmay negative ly regulate the excess inflamma-tion induced by myocardial ischem ia and it is proved by increase plasma apolipoprote in A1 and apoliopoprote in E.

关 键 词：心肌缺血 丹七片 血浆蛋白质组学 

分 类 号：R285.5[医药卫生—中药学]