鞘内注射人前脑啡肽基因修饰HEK293细胞在脑脊液释放L-EK情况及L-EK对骨癌痛的镇痛效果  

Release of L-EK in cerebrospinal fluid induced by intrathecal HEK293 cells modified with human preproenkephalin gene and the analgesic efficacy of L-EK for bone cancer pain

作　　者：潘喻飞[1] 杨保仲[1] 薛朝霞[1] 白凤[1] 胡博[1] 郭旭丽[1] 

机构地区：[1]山西医科大学第一医院麻醉科,太原市030001

出　　处：《中华麻醉学杂志》2014年第8期976-978,共3页Chinese Journal of Anesthesiology

摘　　要：目的 评价鞘内注射人前脑啡肽基因（hPPE）修饰人胚胎肾（HEK293）细胞在脑脊液释放亮氨酸脑啡肽（L-EK）情况及L-EK对骨癌痛的镇痛效果.方法 CIBP雌性SD大鼠40只,采用随机数字表法分为移植组（CIBP＋ hPPE/HEK293）和对照组（CIBP＋ HEK293）,每组20只.于接种癌细胞前1d、接种后8、15、21、25、32和35 d（T17）时测定大鼠热缩爪潜伏期,记录舔/咬移植侧爪次数,以及自由活动时后肢跛行程度.于T4时观测后各组随机取10只大鼠大鼠处死,冰浴收集脑脊液,采用放射免疫法检测hPPE的表达.结果 与对照组比较,移植组大鼠T4-7时热缩爪潜伏期延长,脑脊液L-EK浓度升高,大鼠舔/咬移植侧爪次数及自由活动时后肢跛行程度评分降低（P＜0.01）.结论 大鼠鞘内移植经慢病毒转染hPPE修饰的HEK293细胞株可持续分泌L-EK,有效减轻骨癌痛.Objective To evaluate the release of leucine-enkephalin （L-EK） in the cerebrospinal fluid induced by intrathecal human embryonic kidney （HEK293） cells modified with human preproenkephalin （hPPE）gene and the analgesic efficacy of L-EK for bone cancer pain.Methods Forty CIBP female Sprague-Dawley rats were randomized into transplantation （CIBP＋ hPPE/HEK293,n =20） and control （CIBP ＋ HEK293,n =20）groups using a random number table.At 1 day before inoculation of cancer cells （T1,baseline） and 8,15,21,25,32 and 35 days after inoculation （T2-7）,thermal paw withdrawal latency （TWL） was measured,and the number of licking/biting the claw on the transplantated side and degree of hindlimb limping during free activities were recorded.After observation at T4,10 rats were chosen from each group and sacrificed and the cerebrospinal fluid of rats was collected in an ice bath for detection of hPPE expression using radioimmunoassay.Results Compared with control group,TWL was significantly prolonged,the concentration of L-EK in the cerebrospinal fluid was increased,and the number of licking/biting the claw on the transplantated side and degree of hindlimb limping during free activities were decreased at T4-7 in transplantation group.Conclusion Intrathecal HEK293 cells modified with hPPE gene can continuously secrete L-EK and mitigate bone cancer pain.

关 键 词：癌性疼痛 细胞镇痛 鞘内穿刺 基因修饰 疼痛测定 

分 类 号：R614[医药卫生—麻醉学] R738.1[医药卫生—外科学]