乌司他丁对内毒素致小鼠脑损伤的影响  

Effect of ulinastatin on brain injury induced by lipopolysaccharide in mice

作　　者：李文瑶[1] 朱丹[2] 陶国才[2] 

机构地区：[1]四川省肿瘤医院手术麻醉科,成都市610041 [2]第三军医大学附属西南医院手术麻醉科

出　　处：《中华麻醉学杂志》2014年第8期986-988,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81171034）

摘　　要：目的 评价乌司他丁对内毒素（LPS）致小鼠脑损伤的影响.方法 成年雄性C57小鼠90只,3-4月龄,体重200 - 300 g,采用随机数字表法分为3组（n=30）：对照组（C组）、LPS组和乌司他丁组（U组）.U组腹腔注射乌司他丁10 000 U/kg,L组腹腔注射等容量生理盐水,10 min后,脑室内注射LPS 1 μg/g制备小鼠脑损伤模型.分别于LPS注射后1、3和7d时,取10只小鼠,取尾动脉血样,采用ELISA法测定血浆S100β蛋白和神经元特异性烯醇化酶（NSE）的浓度;然后处死小鼠,取海马组织,测定IL-1β和TNF-α的含量及其mRNA的表达水平.结果 与C组比较,LPS组和U组LPS注射后1、3和7d时血浆S100β蛋白和NSE的浓度、海马组织TNF-α和IL-1β的含量升高,LPS注射后1和3d时海马组织IL-1β和TNF-α的mRNA表达上调（P＜0.01）;与LPS组比较,U组LPS注射后1和3d时血浆S100β蛋白和NSE的浓度、海马组织TNF-α和IL-1β的含量降低,海马组织IL-1β和TNF-α的mRNA表达下调（P＜0.01）.结论 乌司他丁可减轻LPS致小鼠脑损伤,其机制与抑制炎性反应有关.Objective To evaluate the effect of ulinastatin on brain injury induced by lipopolysaccharide （LPS） in mice.Methods Ninety adult male C57 mice,aged 3-4 months,weighing 200-300 g,were randomly divided into 3 groups （n =30 each） using a random number table：control group （C group）,LPS group and ulinastatin group （U group）.Group U received intraperitoneal injection of ulinastatin 10 000 U/kg,while group L received the equal volume of normal saline,and 10 min later brain injury was produced with LPS 1 μg/g injected into the cerebral ventricle.Ten animals were chosen and blood samples were taken for determination of plasma concentrations of S100β protein and neuron-specific enolase （NSE） at 1,3 and 7 days after LPS injection.Then the animals were sacrificed and hippocampal tissues were obtained for determination of interleukin-1β （IL-1 β） and tumor necrosis factor-α （TNF-α） contents and IL-1β mRNA and TNF-α mRNA expression.Results Compared with C group,the plasma concentrations of S100β protein and NSE and contents of IL-1β and TNF-α were significantly increased at 1,3 and 7 days after LPS injection,and IL-1β mRNA and TNF-α mRNA expression was up-regulated at 1 and 3 days after LPS injection in LPS and U groups.Compared with group LPS,the plasma concentrations of S100β protein and NSE and contents of IL-1β and TNF-α were significantly increased,and IL-1β mRNA and TNF-α mRNA expression was down-regulated at 1 and 3 days after LPS injection in group U.Conclusion Ulinastatin can attenuate brain injury induced by LPS in mice,and the mechanism is related to inhibited inflammatory responses.

关 键 词：胰蛋白酶抑制剂 内毒素血症 脑损伤 

分 类 号：R614[医药卫生—麻醉学]