HPLC法同时测定暴马子皮中紫丁香苷、橄榄苦苷的含量  

HPLC simultaneous determination of syringin and oleuropein in Syringae Cortex

作　　者：李明华[1] 程显隆[1,2] 陆以云 余坤子[2] 魏锋[1,2] 吴清[1] 马双成[2] 

机构地区：[1]北京中医药大学,北京100102 [2]中国食品药品检定研究院,北京100050

出　　处：《药物分析杂志》2014年第10期1861-1863,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的：建立测定暴马子皮药材中2个成分（紫丁香苷、橄榄苦苷）的高效液相色谱分析方法。方法：采用Shiseido CAPCELL PAK-C18（250 mm×4.6 mm,5μm）色谱柱,流动相为0.1%甲酸水溶液（A）-乙腈（B）,梯度洗脱（0~45 min,5%B→40%B）,流速1.0 mL·min-1,DAD检测器,检测波长280 nm,柱温25℃。结果：紫丁香苷、橄榄苦苷线性范围分别为0.25~0.74μg（r=0.9987）和0.9~13.1μg（r=1.0000）,平均加样回收率（n=6）分别为97.5%（RSD=1.3%）和98.2%（RSD=1.1%）。结论：不同来源的暴马子皮药材中紫丁香苷、橄榄苦苷含量均存在显著性差异。本方法操作简单快速,重复性好,为暴马子皮药材的质量控制研究奠定了良好的基础。Objective：To develop an HPLC method for the determination of syringin and oleuropein in Syringae Cortex. Methods：Determination was carried out on a Shiseido CAPCELL PAK -C18 （250 mm × 4.6 mm,5 μm） column. The mobile phase was composed of water solvent containing 0.1 % formic acid （ A ） and acetonitrile （ B ） with gradient elution（0 - 45 rain, 5 % B→40% B） at the flow rate of 1.0 mL · min - 1, the detection wavelength was 280 nm for the DAD detector and the column temperature was set at 25 ~C. Results：The calibration curves were linear in the ranges of 0.25 - 0.74 μg for syringin（ r = 0. 9987 ） and 0.9 - 13.1 μg for oleuropein（ r = 1. 0000）. The average recoveries （ n = 6 ） of syringin and oleuropein were 97.5% （ RSD = 1.3% ） and 98.2% （ RSD = 1. 1% ） respectively. Conclusion ： The yields of the two components in different origins exhibit significant differences. This method is simple, quick and repeatable, which lays a good foundation for the quality control of Syringae Cortex.

关 键 词：暴马子皮 紫丁香苷 橄榄苦苷 高效液相色谱 中药材质量控制 

分 类 号：R917[医药卫生—药物分析学]