罗红霉素对香烟烟雾暴露小鼠HDAC2的影响  

The effect of roxithromycin on expressions of HDAC2 in smoking asthma mice

作　　者：徐慧[1] 戴元荣[1] 胡丹红[1] 夏梦玲[1] 

机构地区：[1]温州医科大学附属第二医院呼吸科,浙江温州325027

出　　处：《中国现代医生》2014年第30期1-3,6,共4页China Modern Doctor

基　　金：浙江省自然科学基金(Y2080466);浙江省温州市科技局项目(Y20130351)

摘　　要：目的观察罗红霉素对烟雾暴露哮喘小鼠组蛋白去乙酰化酶(HDAC2)表达的影响,探讨罗红霉素对烟雾暴露哮喘患者可能的治疗作用。方法 SPF级雌性BALB/c小鼠40只,按随机数字表法随机分为对照组(C)、哮喘组(A)、烟雾暴露组(S)、罗红霉素组(R)4组。用卵白蛋白(OVA)致敏和激发的方法制备哮喘和烟雾暴露模型,并用罗红霉素干预。观察各组小鼠肺组织病理超微结构变化,分析支气管肺泡灌洗液(BALF)中的细胞分类计数,并采用Western blot法测定各组小鼠肺组织中HDAC2表达情况。结果哮喘组和烟雾暴露组嗜酸粒细胞计数比例均显著高于对照组(均P<0.01),烟雾暴露组中性粒细胞计数比例显著高于哮喘组(P<0.01);罗红霉素组与烟雾暴露组相比,嗜酸性粒细胞,中性粒细胞比例明显下降(P<0.01)。哮喘组和烟雾暴露组肺组织HDAC2表达均低于对照组,烟雾暴露组低于哮喘组(均P<0.01),罗红霉素组肺组织HDAC2表达高于烟雾暴露组(均P<0.01)。结论罗红霉素可能通过上调HDAC2,改善烟雾暴露小鼠气道炎症。Objective To explore the effect of roxithromycin on airway inflammation of smoking asthma though researching its effect on expressions of histone deacetylase-2（HDAC2） of cigarette smoking asthma mice. Methods Forty female SPF BALB/c mice were divided randomly into 4 groups： control group（C）, asthma group（A）,smoking asthma group（S） and Roxithromycin group（R）. Established asthmatic and smoking asthma models with challenge of OVA and intervention with Roxithromycin. The change of pathematology in different groups were observed. The different cells counts of bronchoalveolar fluid（BALF） were analyzed. The level of HDAC2 in lung homogenate were measured by Western blot. Results The ratios of eosinophil（EOS） to the total cell numbers of BALF in group A and group S were significantly higher than that in group C（both P〈0.01）, while in group S the ratio of neutrophile（NEU） was higher than the group A（P〈0.01）. The ratios of EOS and NEU in group R was significantly decreased than these in group S（P〈0.01）. Western blot showed expressions of HDAC2 in group A and group S were significantly decreased than that in group C（both P〈0.01）, while the HDAC2 level of group S was lower than that of group A（P〈0.01）. The HDAC2 level of group R was higher than group S（P〈0.01）. Conclusion Roxithromycin can decrease the airway inflammation of cigarette smoking asthma mice via increasing the expression of HDAC2.

关 键 词：哮喘 香烟烟雾 组蛋白脱乙酰基酶类 罗红霉素 

分 类 号：R563.9[医药卫生—呼吸系统]