基于磁性微球HCG化学发光免疫检测方法的研究  

Research on HCG Chemiluminescent Immunoassay System Based on Magnetic Microspheres

作　　者：焦艳华[1] 梁媛媛[1] 郭卫强[1] 

机构地区：[1]杭州师范大学材料与化学化工学院,浙江杭州310036

出　　处：《杭州师范大学学报（自然科学版）》2014年第5期495-498,共4页Journal of Hangzhou Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(21005025);杭州市科技发展计划项目(20130533B14);长江学者和创新团队发展计划项目(IRT1231)

摘　　要：以本实验室制备的表面富含羧基的磁性高分子微球作为免疫检测固相载体,将磁性微球与抗体进行偶联,通过优化偶联条件制备得到免疫磁珠,建立基于磁性微球的化学发光免疫检测方法,用来检测HCG.并研究影响化学发光强度的各项因素,绘制了HCG标准曲线,在HCG抗原浓度为0.5~300IU/L范围内线性关系良好,相关系数R=0.990.The magnetic polymer microspheres with carboxyl on the surface made by this lab are used as the solid carriers of the immunoassay .The magnetic microspheres are coupled with antibodies ,immunomagnetic beads are prepared by optimizing the coupling conditions ,and the chemiluminescent immunoassay based on magnetic microspheres is developed for the detection of human chorionic gonadotropin （HCG） .The paper studies each factor which influences the intensity of chemiluminescence ,and draws HCG standard curve .The linear relation has good correlation coefficient R=0 .990 when the HCG antigen concentration is in the range of 0 .5--300IU/L .

关 键 词：磁性高分子微球 化学发光 HCG:检测 

分 类 号：O657.39[理学—分析化学]