三七皂苷对运动性贫血大鼠海马Bcl-2表达的影响  

The influence of PNS on the expression of Bcl-2 of hippocampal in sports anemia rats

作　　者：田阁 白瑶[1] 杨月琴[1] 金丽[1] 

机构地区：[1]武汉体育学院健康科学学院,武汉430079

出　　处：《华中师范大学学报（自然科学版）》2014年第5期730-734,共5页Journal of Central China Normal University：Natural Sciences

基　　金：湖北省教育厅优秀中青年人才项目(Q20083302);2012年度湖北省教育厅优秀中青年科技创新团队项目(T201221)

摘　　要：探讨了三七皂苷对运动性贫血大鼠海马Bcl-2表达的影响.将24只健康雄性4周龄SD大鼠随机分为对照组、运动组和运动给药组,对运动组和运动给药组大鼠进行为期10周的递增负荷跑台运动,并从第5周开始对运动给药组大鼠进行三七皂苷药物干预,对其他各组喂饲等量生理盐水.应用Real-time PCR检测海马组织Bcl-2mRNA的基因表达情况;应用ELISA测定海马组织Bcl-2的蛋白含量.结果表明:运动组海马Bcl-2mRNA表达量和Bcl-2蛋白含量显著低于对照组(P<0.05),运动给药组海马Bcl-2mRNA表达量和Bcl-2蛋白含量显著高于运动组(P<0.05),运动给药组海马Bcl-2mRNA表达量和Bcl-2蛋白含量与对照组相比均无显著性差异.结果提示:三七皂苷能促进运动性贫血大鼠海马Bcl-2的表达,有效抑制大鼠海马神经元凋亡,对运动性贫血造成的脑缺血缺氧性损伤有保护作用.Investigate the influence of PNS on the expression of Bcl-2 of hippocampal in sports anemia rats.The 24 healthy male 4-week-old SD rats were randomly divided into Control group （abbreviated as C group）,sports group （abbreviated as S group） and PNS intervention group （abbreviated as P group）.We drove the S group and P group rats to do increasing load treadmill exercise for ten weeks.And from the fifth week,we gave the P group rats PNS drug intervention,and gave the rats of other group equal volume saline.We measured the Bcl-2 mRNA gene expression in hippocampal by real-time PCR technology,measured Bcl-2 protein content in hippocampal by ELISA technology.The Bcl-2 mRNA gene expression and Bcl-2 protein content in hippocampal of S group were significantly lower than C group （P＜0.05）,the Bcl-2 mRNA gene expression and Bcl-2 protein content in hippocampal of P group were significantly higher than S group （P＜0.05）,the Bcl-2 mRNA gene expression and Bcl-2 protein content in hippocampal of P group were no significant difference with C group.PNS can promote the expression of Bcl-2 in rat hippocampal,inhibit neuronal apoptosis in rat hippocampal neuron and play a protective role in sports anemia caused brain hypoxic-ischemic injury.

关 键 词：运动性贫血 三七皂苷 海马 BCL-2 

分 类 号：G804.23[文化科学—运动人体科学]