基于mtDNA-COⅠ的云南西花蓟马的遗传分析  

Genetic analysis of Frankliniella occidentalis based on mtDNA-COⅠin Yunnan,China

作　　者：沈登荣[1,2] 宋文菲[3] 袁盛勇[1] 田学军[1] 和绍禹[4] 张宏瑞[2] 

机构地区：[1]红河学院生命科学与技术学院,蒙自661100 [2]云南农业大学植物保护学院,昆明650201 [3]云南省农业科学院蚕桑蜜蜂研究所,蒙自661101 [4]云南农业大学东方蜜蜂研究所,昆明650201

出　　处：《植物保护》2014年第5期75-79,共5页Plant Protection

基　　金：公益性行业(农业)科研专项(201103026;200803025);云南省教育厅科学研究基金重点项目(2013Z148);红河学院植物保护硕士点建设项目;云南省高校农作物优质高效栽培与安全控制重点实验室项目

摘　　要：西花蓟马是我国重要的入侵害虫之一,为了解其不同地理种群的遗传分化,测定了云南6个地理种群141个个体的mtDNA-COⅠ基因片段。结果表明:在比对的433bp基因片段中,变异位点19个,共检测出5个共享单倍型,单倍型间出现了明显的遗传分化,证实了云南西花蓟马为一个复合种群。地理种群间的遗传距离较低,遗传距离与地理距离无显著相关性。各地理种群西花蓟马的遗传分化指数Fst为-0.021 19,种群间变异多为负值,表明云南西花蓟马种群遗传变异主要来自于种群内部,种群间未发生明显的遗传分化。Frankliniella occidentalis （Pergande）is an important invasive pest in China. In order to understand the genetic differentiation among six geographical populations of F. occidentalis, mtDNA-COⅠ sequences of 141 in-dividuals were determined. The results showed that there were 19 variable sites in the 433-bp gene fragment. Five shared haplotypes were detected,and these haplotypes had an obvious genetic differentiation,which confirmed that F. occidentalis was a composite population in Yunnan. The genetic distance was low among populations,and there was no significant correlation between genetic distance and geographic distance. The F st value was-0. 021 19 among geographical populations,indicating that genetic variation was mainly derived from within the population,and the genetic differentiation among populations was not significant.

关 键 词：西花蓟马 mtDNA-COⅠ 遗传分化 复合种群 

分 类 号：S436.3[农业科学—农业昆虫与害虫防治]