油菜田看麦娘对精喹禾灵的抗性水平及抗性机理研究  

Resistance level of Alopecurus aequalis to quizalofop-P-ethyl in rape in Hunan Province

作　　者：祝玮玮[1] 刘艺妩[1] 刘祥英[1] 罗坤[1] 柏连阳[1,2] 

机构地区：[1]湖南农业大学农药研究所,长沙410128 [2]湖南省农业科学院,长沙410128

出　　处：《植物保护》2014年第5期80-85,共6页Plant Protection

基　　金：公益性行业(农业)科研专项(201303031)

摘　　要：采用培养皿种子萌发法和盆栽法测定了湖南省境内长沙市、永州市、常德市、益阳市、浏阳市、岳阳市、娄底市7个市州10个地区油菜田看麦娘潜在抗药性种群对精喹禾灵的抗性水平，测定了常德市桃源地区看麦娘潜在抗药性种群以及敏感种群谷胱甘肽-S-转移酶（GSTs）对精喹禾灵的敏感性，对桃源地区看麦娘抗性品系及敏感品系ACCase 基因片段进行扩增和测序，比较了两种生物型的基因序列。培养皿种子萌发法测定结果表明：常德市桃源地区看麦娘抗药性生物型对精喹禾灵的抗性水平最高，抗性倍数为10．50倍，其他地区看麦娘抗性倍数在2．01～7．09倍之间，抗性水平不明显；盆栽法测定结果表明：桃源地区看麦娘抗性倍数最高，为25．30倍，其他地区看麦娘抗性倍数在2．43～9．47倍之间，尚未产生明显抗药性。经精喹禾灵处理后，看麦娘抗药性生物型的 GSTs 活力在第5天明显高于敏感生物型，表明 GSTs 的活性是引起看麦娘对精喹禾灵抗性的重要因子。通过靶标基因片段扩增与 DNA 测序比对发现，抗药性生物型氨基酸序列第93位比敏感生物型多出一个丙氨酸，抗药性的产生与靶标基因的突变是否相关需要进行进一步验证。In order to investigate the resistance level and mechanism of Alopecurus aequalis Sobol.to quizalofop-P-ethyl in rape,10 potential resistant biotypes were collected from 7 cities in Hunan Province and the susceptible biotype was obtained from Nanjing Agricultural University.The resistance level of A.aequalis to quizalofop-P-ethyl was assessed by two methods：seed bioassay and whole-plant bioassay.The enzymology mechanism of Taoyuan Town potential resistant biotype and Nanjing Agricultural University susceptible biotype was studied with the important metabolic enzyme glutathione-s-transferase（GSTs）,and the resistance mechanism at the molecular level was detected by using PCR technology and comparing the amino acid sequences of resistant and sensitive plants.The results of seed bioassay showed that the relative resistance factor of Taoyuan Town in Changde was 10.50,which was the highest.The resistance factor of all of the other potential resistant biotypes ranged from 2.01 to 7.09,which was sensitive to quizalofop-P-ethyl.The results of whole-plant bioassay showed that the rela-tive resistance factor of the biotype from Taoyuan Town in Changde was 25.30,while the factors of other poten-tial resistant biotypes were from 2.43 to 9.47.The GST activity of resistant biotypes was obviously higher than that of the susceptible biotype on the fifth day,indicating that the difference in the metabolic ability of GSTs to quizalofop-P-ethyl was an important reason for the resistance to quizalofop-P-ethyl.The comparison of ACCase sequences between the resistant and sensitive biotypes showed that the amino acid sequence of resistant biotype had one additional Ala,but further verification is needed to figure out the relationships between resistance level and target gene’s mutation.

关 键 词：油菜田 看麦娘 精喹禾灵 抗药性 

分 类 号：S481.4[农业科学—农药学]