香蕉分化芽BBTVDAS-ELISA检测方法的建立和应用  

Establishment and application of DAS-ELISA method for detection of Banana bunchy top virus in banana differentiated shoots

作　　者：周朋[1,2] 余乃通[1] 章绍延[1] 王健华[1] 胡加谊[1] 王祥[1] 梁洁[1] 刘志昕[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所,农业部热带作物生物学与遗传资源利用重点实验室,海口571101 [2]海南大学农学院,海口570228

出　　处：《植物保护》2014年第5期117-120,共4页Plant Protection

基　　金：国家自然科学基金项目(31070131);海南省重大科技项目子课题(ZDZX2013023-1);2012年度海南省研究生创新科研课题(Hys2012-15)

摘　　要：为了解海南海口香蕉分化芽携带香蕉束顶病毒(Banana bunchy top virus,BBTV)的情况,本研究建立了双抗夹心酶联免疫反应(DAS-ELISA)检测法,对来自海南地区组培厂的香蕉分化芽进行检测。检测结果表明,6个品种1 852个香蕉分化芽平均带毒率为2.1%,其中‘皇帝蕉’为1.98%,‘粤科1号’为2.07%,‘广粉蕉’为1.98%,‘大蕉’为6.25%,‘218’为1.89%,‘巴西蕉’为2.25%。为了进一步验证ELISA结果的可靠性,随机选取12个阳性样品提取总DNA进行PCR鉴定。鉴定结果显示,12个样品中11个检测出有BBTV,表明DAS-ELISA方法的准确率较高,与分子检测结果基本一致,可以胜任日常香蕉组培苗BBTV的检测。In order to understand the situation of Banana bunchy top virus （BBTV）infection in banana differentiated shoots in Hainan,1 852 shoot samples from 6 banana varieties were detected by DAS-ELISAmethod.The results showed that the average BBTV-carrying rate was 2.1%,while 1.98% of ‘Huangdi’banana,2.07% of ‘Yueke No.1’banana, 1.98% of ‘Guangfen’banana,6.25% of Musa sapientum ,1.89% of ‘218’banana,and 2.25% of ‘Brazil’banana, respectively.To further confirm the accuracy of DAS-ELISA result,12 BBTV-carrying samples were selected for molec-ular detection by PCR.The results showed that 11 of them were BBTV positive,indicating DAS-ELISA method with the high accuracy can be used for BBTV detection in banana differentiated shoots.

关 键 词：香蕉束顶病毒 DAS-ELISA 病毒检测 

分 类 号：S432.41[农业科学—植物病理学]