黄芪甲苷对大鼠肝缺血/再灌注损伤的保护作用  

Protective effect of Astragaloside IV against liver ischemia and reperfusion injury in rats

作　　者：李铁成[1] 马宝丰[1] 李德生[1] 艾丽丽[1] 艾浩[1] 

机构地区：[1]辽宁医学院附属第三医院,锦州121000

出　　处：《天津中医药》2014年第10期621-623,共3页Tianjin Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81173443);辽宁省教育厅资助科研资助项目(LJQ2011087)

摘　　要：[目的]研究黄芪甲苷对肝缺血/再灌注损伤的保护作用及其机制。[方法]将雄性SD大鼠36只,随机分为3组:假手术组(Sham组)、肝缺血/再灌注组(I/R组)、黄芪甲苷预处理组(AsIV组)。分光光度法测定大鼠血清谷丙转氨酶(ALT)、谷草转氨酶(AST)活性;苏木精-伊红(HE)染色观察肝组织形态学变化,蛋白印迹法(Western blot)检测心肌组织内Bax,Bcl-2蛋白表达。[结果]与假手术组比较,肝I/R组ALT、AST活性显著增高,Bax蛋白表达增加,Bcl-2蛋白表达降低,黄芪甲苷预处理可以降低肝缺血/再灌注损伤引起的ALT、AST活性增高,Bax蛋白表达增加,增加Bcl-2蛋白表达。[结论]黄芪甲苷预处理对肝缺血/再灌注损伤有一定的保护作用,其机制可能与黄芪甲苷抑制肝细胞凋亡有关。[Objective] To investigate the protective effect of Astragaloside IV （As IV） against liver ischemia and reperfusion injury and the underlying mechanism. [Methods] The 36 SD rats were randomly divided into 3 groups： sham operation group （Sham group）, liver ischemia-reperfusion （I/R group） group and As IV group. The content of ALT and AST were measured by spectrophotometric method. Liver pathological changes were observed by HE staining. Bax and Bcl-2 protein expression was determined by Western blot. [Results] Compared with sham group, the content of ALT and AST was significantly increased, while the Bcl-2 protein expression decreased and the Bax expression increased. Pretreatment of As IV before liver I/R could decrease the content of ALT and AST, Bax expression, and increase the Bcl-2 protein expression （P〈0.05）. [Conclusion] As IV pretreatment has a protective effect against liver ischemia reperfusion injury, which is partially finished via inhibiting liver cell apoptosis.

关 键 词：黄芪甲苷 肝缺血/再灌注损伤 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]