十溴联苯醚对青春期雄性小鼠精子数及睾酮的影响  

Influence of BDE-209 on sperm count and testosterone of adolescent male mice

作　　者：王汉永[1] 翟金霞[1] 刘婷[1] 戴瑞雪[1] 任征[1] 丁承辉[1] 林浩飞 

机构地区：[1]安徽医科大学公共卫生学院劳动卫生与环境卫生学系,安徽合肥230032

出　　处：《中华疾病控制杂志》2014年第10期991-994,共4页Chinese Journal of Disease Control & Prevention

基　　金：安徽省自然科学基金(1408085MH192);安徽医科大学博士基金(XJ201201)

摘　　要：目的探讨十溴联苯醚(decabromodiphenylether,BDE-209)对青春期雄性ICR小鼠的生殖毒性。方法青春期雄性ICR小鼠随机分为6组:对照组(生理盐水),DMSO组,BDE-209处理组(100,200,300,500 mg/kg BW)。小鼠经口染毒7周后处死,称量小鼠睾丸、附睾重量并计算脏器系数。采用放射免疫法检测小鼠血清中睾酮水平,采用ELISA法检测小鼠血清中cAMP水平。结果与对照组相比,DMSO组未见明显生殖损害(均有P>0.05),而BDE-209处理组睾丸重和附睾重、睾丸脏器系数和附睾脏器系数均显著降低(均有P<0.05);与对照组相比,BDE-209处理组小鼠精子数目亦减少(均有P<0.05)。进一步研究发现,BDE-209处理后,小鼠血清cAMP浓度和睾酮浓度低于对照组(均有P<0.05)。结论 BDE-209暴露青春期雄性小鼠导致小鼠睾酮合成降低,精子合成减少,损伤青春期雄性小鼠生殖功能。Objective To discuss the reproductive toxicity of BDE-209 to the adolescent male ICR mice. Meth- ods The adolescent male ICR mice were randomly divided into 6 groups： control group （NS）, DMSO group, and BDE- 209 treatment groups（ 100,200,300,500 mg/kg BW）. Mice were orally given theagent for seven weeks, and were execu- ted. The weight of the mouse testis and epididymis were measured and their organ coefficients were calculated. The concen- tration of testosterone in sera was detected by RIA method. The concentration of cAMP in sera was detected by ELISA. Re- sults Compared with the control group, the DMSO group showed no obvious reproductive damage （ all P 〉 0. 05 ）, the weight of testis and epididymis and organ coefficients of testis and epididymis in BDE-209 treatment groups were significant- ly reduced （ all P 〈 0.05 ）. Compared with the control group, the mice sperm count was also significantly reduced in BDE- 209 treatment groups（ all P 〈0.05 ）. Additionally, the mice serum concentration of cAMP and testosterone concentration were significantly lower than those of the control group （ all P 〈 0. 05 ）. Conclusions The adolescent male mice exposed to BDE-209 lead to reducing of testosterone synthesis, and sperm synthesis and damage to the mice reproductive function.

关 键 词：雌激素类 生殖毒性 睾酮 

分 类 号：R994[医药卫生—毒理学]