高效亲和色谱法测定牛初乳加钙咀嚼片中免疫球蛋白IgG的含量  

Determination of IgG in Bovine colostrum plus calcium Chewable Tablet by HPAC

作　　者：邢俊波[1] 曹红[1] 陈玉敏[1] 水彩红[1] 单婷婷[1] 胡丹[1] 姜春来[1] 靳守东[1] 

机构地区：[1]中国人民解放军总后勤部卫生部药品仪器检验所,北京100071

出　　处：《中国野生植物资源》2014年第5期23-25,共3页Chinese Wild Plant Resources

基　　金：国家重大科学仪器设备开发专项项目(2012YQ18013208)

摘　　要：目的：建立高效亲和色谱法（HPAC）测定牛初乳加钙咀嚼片中免疫球蛋白IgG含量的方法.方法：采用HI-Trap Protein G HP亲和色谱柱（1 mL）色谱柱,以0.05 mol/L磷酸盐缓冲液和0.05 mol/L的甘氨酸-盐酸缓冲液为流动相,流速0.4 mL＆#183;min-,检测波长280 nm,柱温30 ℃.结果：IgG含量在0.1502 ～0.7512 mg/mL浓度范围内与峰面积呈良好的线性关系（r =0.999 9）;平均回收率为98.5％,RSD为1.2％（n=6）.结论：方法简便、快速,重现性好.可作为牛初乳加钙咀嚼片的质量控制方法.Objective： To establish a high performance affinity chromatography （HPAC） method for de- termination of IgG in Bovine colostrum plus calcium Chewable Tablet. Methods： Chromatography was per- formed on HI-Trap Protein G HP affinity column. The 0.05 mol/L PBS and 0.05 mol/L glycin-hydrochlo- ric acid buffer solution was used as mobile phase. The flow rate was 0.4ml/min. Detective wavelength was 280 nm. Results：The calibration curves of IgG was linear within the range of 0. 150 2 ～ 0. 751 2 rag/ mL. The average recovery was 98.5% ,and RSD was 1.2% （n =6）. Conclusion： This method is sim- ple, fast, and reproducible. It can be used for the determination of the preparation including IgG.

关 键 词：牛初乳加钙咀嚼片 免疫球蛋白IGG 高效亲和色谱 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]