重连口服液对内毒素致热新西兰兔解热作用及对血清TNF-α、IL-1β、IL-6表达水平的影响  

Antipyretic Action of Chonglian Oral Solution in New Zealand Rabbits with Endotoxin-Induced Fever and Its Effects on Expression of Tumor Necrosis Factor-α,Interleukin-1β,and Interleukin-6

作　　者：李雪苓[1] 韩宁林[1] 徐桂琴[1] 韩燕全[1] 李晗[1] 王凯[1] 范泉[1] 李忠志[1] 杨德玉[1] 李泽庚[1] 

机构地区：[1]安徽中医药大学第一附属医院,安徽合肥230031

出　　处：《安徽中医药大学学报》2014年第5期63-65,共3页Journal of Anhui University of Chinese Medicine

基　　金：安徽高校省级自然科学研究项目(KJ2011Z226);国家中医药管理局重点学科开放基金项目(2011fbxk017B)

摘　　要：目的观察重连口服液的解热作用及其对炎性递质的影响。方法将36只新西兰兔随机分为6组,即正常组,模型组,重连口服液低、中、高剂量组和布洛芬组,每组6只。连续给药4d,采用大肠杆菌内毒素注射法复制发热模型。于注射后0.5、1.0、2.0、3.0、4.0h分别观察各组兔直肠温度的变化,采用放射免疫法测定注射后4h各组血清肿瘤坏死因子-α(tumor necrosis factor alpha,TNF-α)、白细胞介素-1β(interleukin-1beta,IL-1β)和白细胞介素-6(interleukin-6,IL-6)水平。结果重连口服液低、中、高剂量组在注射后1.0、2.0、3.0、4.0h直肠温度升高值显著低于模型组(P<0.05,或P<0.01),其解热作用较布洛芬组起效慢,但退热维持时间较长。重连口服液高剂量组血清IL-1β、TNF-α、IL-6水平均较模型组显著降低(P<0.05,或P<0.01)。结论重连口服液具有显著的退热作用,其作用机制可能与其抑制炎性因子的释放有关。Objective To investigate the antipyretic action of Chonglian oral solution in New Zealand tabbits with endotoxin induced fever and to observe its effects on the expression of tumor necrosis factor-α （TNF-α）,interleukin-1β （IL-1β）,and interleukin-6 （IL-6）.Methods Thirty-six New Zealand rabbits were randomly and equally divided into normal group,model group,low,medium,and high-dose Chonglian oral solution groups,and ibuprofen group.After 4 d administration of respective drugs,these rabbits were injected with Escherichia coli endotoxin to induce a fever model.Rectal temperature was measured at 0.5,1.0,2.0,3.0,and 4.0 h after injection.Serum levels of TNF α,IL-1β,and IL-6 were determined by radioimmunoassay at 4.0 h after injection.Results At 1.0,2.0,3.0,and 4.0 h after injection,the low,medium,and high dose Chonglian oral solution groups had significantly lower increases in rectal temperature than the model group （P＜0.05 or P＜0.01） ; Chonglian oral solution had a slower onset of antipyretic action than ibuprofen,but had a longer antipyretic effect.Compared with the model group,the high dose Chonglian oral solution group had significantly reduced serum IL-1β,IL-6 and TNF-α levels （P＜0.05 or P＜0.01）.Conclusion Chonglian oral solution has a significant antipyretic effect,and its action mechanism may be related to the inhibition of release of inflammatory factors.

关 键 词：发热 重连口服液 解热 炎性递质 

分 类 号：R285.5[医药卫生—中药学]