高效液相色谱法同时测定脑络欣通复方提取物中三七皂苷和人参皂苷含量  

Simultaneous Determination of Notoginsenoside and Ginsenosides in Compound Naoluoxintong Extracts by High-performance Liquid Chromatography

作　　者：方文悠[1,2] 胡容峰[2,3,4] 王斌[2] 孙备[5] 王章姐[6] 

机构地区：[1]安徽中医药大学研究生部,安徽合肥230038 [2]省部共建新安医学教育部重点实验室,安徽合肥230038 [3]安徽省"115"新安医药研究与开发创新团队,安徽合肥230038 [4]安徽省中药研究与开发重点实验室,安徽合肥230038 [5]安徽省药物研究所,安徽合肥230022 [6]安徽新华学院,安徽合肥230088

出　　处：《安徽中医药大学学报》2014年第5期89-92,共4页Journal of Anhui University of Chinese Medicine

基　　金：国家科技支撑计划项目(2012BAI26B03);国家自然科学基金项目(81274100);安徽省学术和技术带头人及后备人选科研活动经费资助项目(皖人社秘2011-381号-26)

摘　　要：目的 建立同时测定中药复方脑络欣通提取物中三七皂苷R1,人参皂苷Rb1、Rg1、Rd、Re的高效液相色谱法分析方法,为脑络欣通制剂的质量控制提供依据.方法 采用Welch Materials C8色谱柱（4.6mm×250 mm,5 μm）,选择乙腈-水作为流动相,进行梯度洗脱,流速1.2 ml/min,检测波长为203 nm,柱温20℃.结果 三七皂苷R1,人参皂苷Rb1、Rg1、Rd、Re分别在0.081 7～0.980 4（r=0.999 9）、0.315 7～3.788 4（r=0.999 9）、0.300 0～3.600 0（r=0.999 6）、0.077 7～0.932 4（r=0.999 9）、0.074 6～0.895 2（r=0.999 9）μg与峰面积呈良好的线性关系;平均回收率分别为99.72％、99.65％、101.23％、99.38％、100.62％（n=6）.结论 所建立的高效液相色谱方法简单方便、准确可靠,5种物质分离度佳、重复性好,可用于中药复方脑络欣通的质量控制.Objective To establish a high performance liquid chromatography （HPLC） method for simultaneous determination of notoginsenoside R1 and ginsenosides Rb1,Rg1,Rd,and Re in compound Naoluoxintong extracts and to provide a methodological basis for the quality control of compound Naoluoxintong.Methods HPLC was performed on an Welch Materials C8 column （4.6 mm × 250 mm,5 μm） with a mobile phase of acetonitrile water for gradient elution at a flow rate of 1.2 ml/min,a detection wavelength of 203 nm,and a column temperature of 20 ℃.Results The content of notoginsenoside R1 and ginsenosides Rb1,Rg1,Rd,and Re showed a good linear relationship with the peak area within 0.081 7-0.980 4 μg （r =0.999 9）,0.315 7-3.788 4 μg （r=0.999 9）,0.300 0-3.600 0μg （r=0.999 6）,0.077 7-0.932 4 μg （r=0.999 9）,and 0.074 6-0.895 2μg （r=0.999 9） ; the average recovery rates were 99.72％,99.65％,101.23％,99.38％,and 100.62％ （n=6）.Conclusion The established HPLC method is simple,convenient,accurate,reliable,and repeatable.With this method,notoginsenoside R1 and ginsenosides Rb1,Rg1,Rd,and Re can be well separated,and this method can be used for the quality control of compound Naoluoxintong.

关 键 词：脑络欣通 三七皂苷 人参皂苷 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]