慢性运动性中枢疲劳大鼠血清色氨酸与亮氨酸的测定价值  

Levels of leucine and tryptophan in rats' serum associated with chronic exercise central fatigue

作　　者：刘占东[1] 吴岩珏 王得新[3] 薛启蓂[3] 张拥波[3] 刘天会[4] 李任[5] 张健[1] 杨伊姝[1] 谢敏豪[6] 

机构地区：[1]首都医科大学附属北京友谊医院医保中心,北京100050 [2]美国疾病与预防控中心 [3]首都医科大学附属北京友谊医院神经内科,北京100050 [4]首都医科大学附属北京友谊医院实验中心,北京100050 [5]首都医科大学附属北京友谊医院药理研究室,北京100050 [6]北京体育大学,北京100084

出　　处：《临床和实验医学杂志》2014年第20期1663-1665,共3页Journal of Clinical and Experimental Medicine

基　　金：国家自然科学基金专项基金(中枢性疲劳时有关5-HT能系统的多向性研究:应用生化;电生理;MRS方法对临床病例及实验动物的观察;SN 81041019);国家科技支撑项目("运动促进体质健康关键技术的研究与应用"分课题"制定有效运动负荷方法与评价等级的研究";SN 2012BAK21B02);北京市保健科研课题(慢性失眠对干部人群脑功能影响的研究;SN京13-03)资助

摘　　要：目的通过对于慢性运动性中枢疲劳模型大鼠血清色氨酸及亮氨酸含量测定,探讨色氨酸及支链氨基酸在慢性运动性中枢疲劳调控机制方面的变化规律。方法建立大鼠慢性运动性中枢疲劳模型;应用高效液相色谱-质谱的研究方法对大鼠血清色氨酸及亮氨酸进行测定。结果与正常饲养大鼠比较发现,奔跑条件下运动性中枢疲劳大鼠血清中色氨酸水平明显降低,差异有显著性(P<0.05);亮氨酸的水平无统计学差异(P>0.05);亮氨酸/色氨酸明显升高,差异有显著性(P<0.05)。结论慢性运动性中枢疲劳时大鼠血中色氨酸表现为明显下降的趋势,支链氨基酸亮氨酸与其比值明显升高可能进一步抑制其进入中枢,调控中枢性疲劳的发生和发展,该变化可能是机体为适应慢性运动性中枢疲劳状态的一种自我调控反应。Objective The aim of this paper is to analyze mechanisms of motion regulation of tryptophan and branched-chain in chronic ex-ercise central fatigue,through the analysis of tryptophan and leucine in serum in ratˊs model of chronic exercise central fatigue. Methods To set up the ratˊs model of chronic exercise central fatigue. To test the tryptophan and leucine in Ratsˊserum with HPLC-MS. Results Compared with the control rats,the level of tryptophan in exercise central fatigue rats reduces sharply and there is a significant difference（ P 〈0. 05）. There is no sta-tistical difference in the level of leucine. Leu/Trp rises sharply with significant difference（ P 〈0. 05）. Conclusion There is a clear trend of decli-ning in the level of tryptophan in the blood of rats under training with chronic exercise central fatigue. Because of the rising level of the ratio between leucine and tryptophan in branched-chain amino acids. It refines tryptophan to entering the central nerves system,regulating the occurring and de-velopment of the central fatigue. This alternation is possibly a self-regulation reaction in order to adapt to chronic exercise central fatigue.

关 键 词：大鼠 中枢性疲劳 训练 色氨酸 亮氨酸 支链氨基酸 

分 类 号：R87[医药卫生—运动医学]