南岭国家濒危植物长柄双花木遗传多样性研究  

Genetic diversity of national endangered plant Disanthus cercidifolius var. longipes in Nanling

作　　者：谢国文[1] 李丽卡 赵俊杰[1] 郑毅胜[1] 李象钦 张嘉茗[1] 刘萍萍[1] 李海生[2] 

机构地区：[1]广州大学生命科学学院,广东广州510006 [2]广东第二师范学院生物与食品工程学院,广东广州510303

出　　处：《广州大学学报（自然科学版）》2014年第4期47-52,共6页Journal of Guangzhou University:Natural Science Edition

基　　金：国家自然科学基金资助项目(31270259;30970191;30470146);全国大学生"挑战杯"课外科技活动项目

摘　　要：应用ISSR分子标记技术,对长柄双花木（Disanthus cercidifolius var.longipes）5个南岭野生种群的遗传多样性进行了初步研究.8条ISSR引物扩增出101条带,其中64条具多态性,多态位点百分率为63.37%.总的Nei＇s基因多样性指数为0.285 9,种群内基因多样性（Hs）为0.274 1,长柄双花木在物种水平上遗传多样性水平较低.在种群水平上,种群多态位点百分率在60%~75%之间,Nei＇s基因多样性指数为0.269 5~0.282 4,Shannon信息多态性指数为0.384 5~0.399 9.道县种群和莽山种群的遗传多样性水平较高.种群间的基因分化系数为0.041 4,表明长柄双花木绝大多数遗传变异发生在种群内的个体间（95.86%）.UPGMA聚类分析结果表明：5个野生种群未按地理位置进行聚类,连州2个种群较为特殊,先与江西井冈山种群聚在一起,具体原因有待进一步研究.The genetic diversity of Disanthus cercidifolius var.longipes from five wild populations in Nanling was studied by means of ISSR markers , in order to provide theoretical basis for the protection and sustainable utilization of the endangered plant .Eight ISSR primers gave rise to 101 discernible bands , of which 64 （ 63.37%） were polymorphic.The total Nei＇s genetic diversity index was 0.285 9, genetic diversity within populations （ Hs ） was 0.274 1, indicating a relatively lower genetic variation at the species level .At the population level , the percentage of polymorphic bands were 60%-75%, Nei＇s genetic diversity index were 0.269 5-0.282 4 , Shannon＇s information index were 0.384 5-0.399 9 .Daoxian population and Mangshan population had higher genetic diversity levels .Genetic differentiation among populations was 0.041 4 , indicating a large proportion of genetic diversity （ 95.86%） was resided among individuals within populations .The UPGMA cluster analysis showed that the five populations were not basically clustered according to their geographic distances , it was surprising that the two Lianzhou populations （ LZX and LXD ） clustered firstly with Jinggangshan population （ JGS） , the reason should be explored further .

关 键 词：长柄双花木 金缕梅科 遗传多样性 ISSR 

分 类 号：Q346.5[生物学—遗传学] Q789