基于生物质谱的定量蛋白质组学研究进展  

Advancements in Bio-Mass Spectrometry Based Quantitative Proteomics

作　　者：张磊 尹红锐 张莹[1] 陆豪杰[1] 

机构地区：[1]复旦大学生物医学研究院与化学系,上海200032

出　　处：《分析测试技术与仪器》2014年第3期139-147,共9页Analysis and Testing Technology and Instruments

基　　金：国家重点基础研究发展计划(2012CB910602);国家自然科学基金项目(21025519;21335002);上海高校特聘教授(东方学者跟踪)岗位计划;上海市重点学科发展基金项目(B109)资助

摘　　要：随着蛋白质组研究和生物质谱技术的发展,大规模的蛋白质组相对定量和绝对定量已经成为了解生命活动进程、疾病发生发展过程以及生物标志物筛选和验证的重要策略,并形成蛋白质组学研究领域的一个重要分支:定量蛋白质组学.综述了近年来定量蛋白质组学的研究进展,并对其中的关键技术进行讨论.With the development of proteomics and biomass spectrometry, large scale quantitative proteomics has become an important aspectof proteomics. Quantitative proteomics plays important roles in deciphering the biological progress, understanding the development of the disease, and discovering as well as verifying biomarkers for the diagnosis and therapy of diseases. In this review, the status of quantitative proteomics has beenaddressed. The principles, advantages and disadvantages, as well as theapplications of various quantitative proteomics strategieswere summarized and discussed.

关 键 词：生物质谱 蛋白质组 相对定量 绝对定量 

分 类 号：O657.6[理学—分析化学]