不同施肥处理对茶树根际细菌多样性的影响  

Effect of Fertilizer on Tea Plant Rhizosphere Bacteria Diversity

作　　者：罗毅[1] 苏有健[1] 张永利[1] 孙力[1] 胡善国[1] 王烨军[1] 廖万有[1] 

机构地区：[1]安徽省农业科学院茶叶研究所,安徽黄山2450000

出　　处：《中国农学通报》2014年第25期177-183,共7页Chinese Agricultural Science Bulletin

基　　金：国家茶叶产业技术体系土壤肥料岗位项目"茶园土壤质量提升技术创新研究"(CARS-23-07B);安徽省农业科学院科技创新基金项目"茶树根际芽孢杆菌定殖机理的研究"(13B0808);安徽省农业科学院高层次人才培养及引进项目"茶树根内内生细菌多样性的研究"(HB2013006)

摘　　要：采用变性梯度凝胶电泳(denaturing gradient gel electrophoresis,DGGE)方法测定茶树根际和根围土细菌群落丰富度,结合土壤pH和C/N分析影响因子,并通过16S rRNA基因序列分析特异菌株的系统发育地位,最后利用比色法对茶树根系生长素(IAA)含量进行定量检测,探讨不同施肥处理下茶树根际和根围土细菌的群落的变化以及对茶树根系生长的促进影响。结果表明:有机肥能明显增加土壤C/N,C/N与细菌丰富度正相关,相关系数(r)为0.93(P<0.05);有机肥与化肥(7:3)的处理,根际细菌多样性最高,有机肥与化肥(95:5)的处理,根围土中的细菌多样性最高;化肥和有机肥会影响特异菌种的定殖效率;茶树根生长素(IAA)定量测定发现,根际细菌多样性最高的处理,根系IAA的含量也最高。研究表明有机肥施用能提高节土壤C/N和微生物区系多样性但对土壤pH的提升有限;C/N与茶树根际细菌群落多样性正相关。Denaturing gradient gel-electrophoresis （DGGE） method was employed to determine tea plant rhizosphere and body soil bacteria community diversities with soil pH and C/N, to investigate the effect of different fertilization treatments. More detailed analysis of 16S rRNA gene sequences to determine the phylogenetic position of specific strains was conducted. In addition, root auxin （IAA） quantity was detected by colorimetry. Results showed that organic fertilizer could significantly increase soil C/N, which was positively correlated to bacteria community, and the correlation coefficient （r） was 0.93 （/）〈0.05）. Among different treatments, bacteria diversity was the highest in the organic and chemical fertilizer （7：3） treatment （rhizosphere） and （95：5） treatment （soil） respectively. Bacteria 16S rRNA band gene sequences showed both chemical fertilizer and organic fertilizer would affect the efficiency of specific bacterial colonization. The results also showed the highest bacteria diversity treatment also contained the highest content of IAA in tea root. In conclusion, organic fertilizer can elevate soil C/N and microbial community diversity and bacterial diversity has positive relationship to soil C/N.

关 键 词：茶树 施肥 C/N 细菌多样性 IAA 

分 类 号：Q939.96[生物学—微生物学]