低磷胁迫对玉米自交系齐319和其突变体齐319-96叶片光合作用、叶绿素荧光特性的影响  

Effect of Low Phosphorous Stress on Photosynthesis and Chlorophyll Fluorescence Characteristics of Maize Inbred Lines Qi319 and low-phosphorus-tolerant Mutant Qi319-96

作　　者：刘寒寒[1] 钟文 张可炜[1] 

机构地区：[1]山东大学生命科学学院/植物细胞工程与种质创新教育部重点实验室,济南250199 [2]山东种子管理总站,济南250199

出　　处：《中国农学通报》2014年第27期21-28,共8页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金资助项目"应用蛋白质组学方法解析耐低磷玉米高光效的分子机制"(31172028);山东省自然科学基金自目"磷高效利用玉米杂交种耐低磷机制的研究"(ZR2010CM053)

摘　　要：为了解低磷胁迫下不同磷利用效率玉米叶片在光能的吸收、分配和过剩激发能的耗散方面的差异,以利用细胞工程技术获得的不同磷利用效率玉米齐319和Qi319-96为材料,采取水培的方法,研究了低磷(KH2PO45μmol/L,-P)和正常供磷(KH2PO41000μmol/L,+P)2个供磷水平对玉米叶片的光合作用及叶绿素荧光参数的影响。结果表明:低磷胁迫下突变体齐319-96叶片的光能转换和电子传递效率高于玉米自交系齐319;突变体齐319-96叶片吸收的光能用于光化学反应部分显著高于齐319;通过天线热耗散部分的能量低于齐319;二者通过其他途径耗散过剩光能的部分差异不显著。叶片通过活性氧清除系统对过剩光能耗散作用在自交系齐319和突变体齐319-96间差异不显著。低磷胁迫下与齐319相比,突变体齐319-96叶片中的无机磷含量显著提高。与自交系齐319相比低磷下自交系齐319-96具有更高的光能利用效率和碳同化速率,推测不同磷效率玉米自交系之间光合特性差异与叶片中无机磷含量的差异有关。To better understand the differences on light absorption, distribution and surplus energy dissipation between two different phosphorus efficiency maize genotypes under low phosphorus, hydroponics was conducted with the low phosphorus tolerant mutant Qi319-96 and wild type Qi319 as the test materials. The leaf photosynthesis and chloroplast fluorescence characteristics were detected between two inbred lines grown under P-deficient （KH2PO4 5 μmol/L, -P） and P-sufficient （KH2PO4 1000 p, mol/L, ＋P） conditions. The results showed that the light energy conversion efficiency and electron transport rate （ETR） of Qi319-96 were higher than that of Qi319. The light absorbed by maize that entered into the photochemical reaction in Qi319-96 was higher than that in Qi319, whereas thermal energy dissipation through antenna pigments was more weakened. The surplus energy that dissipated by ROS-scavenging system had no significant diversity between Qi319-96 and Qi319 under phosphorus starvation. Inorganic phosphorus content was higher in Qi319-96 in comparison with Qi319 under low phosphorus stress. Compared to Qi319, Qi319-96 possessed higher efficiency of light energy conversion and carbon assimilation. These results indicated that different inorganic phosphorus concentration of leaf may be the major cause for the variation of photosynthetic characteristics between Qi319- 96 and Qi319.

关 键 词：玉米 突变体 光合作用 叶绿素荧光 

分 类 号：Q945.11[生物学—植物学]