6-BA对养分胁迫下水稻幼苗根系生长影响的蛋白质组学分析  

Proteomics Analysis of 6-BA on Rice Seedlings Roots Growth Under Nutrient Stress Condition

作　　者：唐秀英[1] 邵彩虹 余南生 王会民[1] 龙起樟 黄永兰[1] 芦明[1] 谢金水 万建林[1] 

机构地区：[1]江西省农业科学院超级水稻研究发展中心,南昌330200 [2]江西农业科学院土壤肥料与资源环境研究所,南昌330200 [3]星子县农业局,江西星子332800

出　　处：《中国农学通报》2014年第27期202-207,共6页Chinese Agricultural Science Bulletin

基　　金：国家科技支撑计划资助项目"长江中游南部(江西)双季稻丰产高效技术集成研究与示范"(2006BAD02A04);江西省农业科学院创新基金"养分胁迫下6-苄基腺嘌呤延缓水稻衰老的分子机理研究"(2008CBS003)

摘　　要：为了揭示养分胁迫下6-BA维持水稻幼苗根系生长的分子机理,本研究采用蛋白组学分析技术,对养分胁迫下6-BA培养的水稻幼苗根系的蛋白质组差异表达进行比较分析。经过双向凝胶电泳分离,从水稻幼苗根系中获得20个响应6-BA的差异表达蛋白质,其中14个蛋白质得到质谱鉴定,这14个蛋白质按其功能可以分为四大类:包括7个参与呼吸代谢的蛋白质,4个参与生长发育的蛋白质,2个参与逆境胁迫的蛋白质以及1个未知功能的蛋白质。分析结果表明,养分胁迫下,6-BA诱导了根系中参与呼吸代谢及生长发育相关蛋白质的表达量上调,增强根系的呼吸作用,促进根系的生长。In order to reveal the molecular mechanism of 6-BA on maintaining the growth of rice seedlings roots under nutrition stress, the proteomics approach was used to research the differential protein expression profile in rice seedlings roots. Proteins were separated by two-dimensional gel eleetrophoresis, twenty differentially expressed proteins that induced by 6-BA were obtained from roots, among which 14 proteins were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry. These 14 proteins were categorized into four groups according to functions： 7 proteins were associated with respiration, 4 proteins were associated with growth, 2 proteins were associated with adversity stress and 1 protein was putative protein. The functions of these 14 proteins were analyzed and the conclusion was drawn from the following results： under nutrient stress conditions, 6-BA increased the expression abundance of proteins associated with respiration and growth, enhanced the roots ＇ respiration, and promoted the growth of root.

关 键 词：水稻幼苗 6-BA 养分胁迫 蛋白质组学 根系 

分 类 号：Q946.1[生物学—植物学] S511.01[农业科学—作物学]