牡丹丹皮酚对禾谷丝核菌菌丝体超微结构与主要成分含量的影响  

Effects of Peony Paeonol on Mycelial Ultrastructure and Content of Main Composition in Mycelia of Rhizoctonia cerealis

作　　者：成玉梅[1] 康业斌[2] 

机构地区：[1]河南科技大学化工与制药学院,河南洛阳471023 [2]河南科技大学林学院,河南洛阳471003

出　　处：《中国农学通报》2014年第27期270-274,共5页Chinese Agricultural Science Bulletin

基　　金：河南省科技攻关重点基金项目"牡丹真菌病害的rDNAITS序列分析鉴定及防控关键技术研究"(102102110090)

摘　　要：为了揭示丹皮酚的抗菌机理,试验采用扫描电镜和透射电镜,研究了丹皮酚对禾谷丝核菌菌丝形态和超微结构的影响,采用分光光度法测定了丹皮酚对禾谷丝核菌菌丝体可溶性蛋白、还原糖、几丁糖含量的影响。结果表明,丹皮酚处理12 h后,菌丝顶端不规则膨大,分枝肿大或顶端缢缩,24 h后,菌丝顶端、分枝顶端或整个菌丝塌陷。菌丝细胞超微结构的变化包括细胞膜内突,细胞壁不规则加厚,加厚部位出现不明内含物,细胞内线粒体、液泡和脂肪粒等细胞器紊乱,在坏死的菌丝细胞中形成子菌丝。丹皮酚使禾谷丝核菌菌体可溶性蛋白与还原糖含量显著降低,且含量水平与处理浓度呈负相关;丹皮酚使菌体几丁糖含量显著升高,且含量水平与处理浓度呈正相关。In order to reveal the antibacterial mechanism of paeonol, the effects of paeonol on morphology and uhrastructure of mycelial cell of Rhizoctonia cerealis were studied by scanning electron microscopy（SEM） and transmission electron microscopy（TEM）, and the effects of paeonol on contents of soluble protein, reducing sugar and chitosan in mycelia of Rhizoctonia cerealis were determined by spectrophotometry. The results showed that after the mycelium were treated with paeonol 12 h, hyphal tips swelled irregularly and hyphal branches swelled or had constriction, and after 24 h, hyphal tips, hyphal branches or the mycelium collapsed. The structural alterations of hyphae included irregular swelling of the hyphal cell membrane, irregular thickening of the hyphal cell walls, the unknown inclusions appeared in the thickened part, the organelles, such as mitochondrions, vacuoles and fat granule, became disordered, the daughter hyphae formed in the necrotic hyphal cells. The contents of soluble proteins and reducing sugar in the mycelia of Rhizoctonia cerealis remarkably decreased under the influence of paeonol, and their levels were negative correlated to the concentration of paeonol solusion. The content of chitosan remarkably increased, and its level was positive correlated to the concentrations of paeonol solusion.

关 键 词：丹皮酚 禾谷丝核菌 菌丝体 超微结构 

分 类 号：S432.23[农业科学—植物病理学]