PCR-SSP法、FCM法及MLCT法检测强直性脊柱炎患者HLA-B_(27)对比分析  

Contrastive Analysis of the Value of Detecting HLA-B27 with PCR-SSP Method,FCM Method and MCLT Method in the Diagnoses of Ankylosing Spondylitis

作　　者：于莉[1] 

机构地区：[1]海阳市第三人民医院,山东海阳265100

出　　处：《风湿病与关节炎》2014年第10期13-15,19,共4页Rheumatism and Arthritis

摘　　要：目的:探讨序列特异性引物——聚合酶链反应(PCR-SSP)法、流式细胞仪(FCM)法及微量淋巴细胞毒(MLCT)法检测血清人类白细胞抗原-B27(HLA-B27)对强直性脊柱炎的诊断价值。方法:将58例确诊的强直性脊柱炎患者作为观察组,58例健康体检者作为对照组,分别采用PCR-SSP法、FCM法及MLCT法检测血清HLA-B27,分析3种方法诊断强直性脊柱炎的灵敏度、特异度、阳性预测值及阴性预测值。结果:PCR-SSP法、FCM法、MLCT法检测血清HLA-B27诊断强直性脊柱炎的灵敏度分别为96.55%、93.10%、82.76%,特异度分别为94.83%、91.38%、93.10%;PCR-SSP法、FCM法的灵敏度显著高于MLCT法,差异有统计学意义(P<0.05);3种方法均有较高的特异度,差异无统计学意义(P>0.05);PCR-SSP法具有较高灵敏度和特异度。阳性预测值分别为94.92%、91.53%、92.31%,阴性预测值分别为96.49%、92.98%、84.38%。3种方法均有较高的阳性预测值,差异无统计学意义(P>0.05);PCR-SSP法、FCM法的阴性预测值显著高于MLCT法,差异有统计学意义(P<0.05);PCR-SSP法具有较高的阳性预测值和阴性预测值。结论:3种检测方法对诊断强直性脊柱炎均有较高的特异度和阳性预测值,PCR-SSP法具有较高灵敏度、特异度、阳性预测值和阴性预测值。Objective：To investigate the value of detecting HLA-B27 with PCR-SSP method,FCM method and MCLT method in the diagnosis of ankylosing spondylitis（AS）.Methods：Selected 58 cases of AS patients as the observation group and 58 healthy subjects as the control group.HLA-B27 was detected respec-tively using the PCR-SSP method,FCM method and MLCT method,analyzing the diagnoses of the sensitivi-ty,speciifcity,positive predictive value and negative predictive value of AS with 3 methods.Results：By detecting HLA-B27 with PCR-SSP method,FCM method and MLCT method,the sensitivity of AS were 96.55%,93.10%and 82.76%,and speciifcity was 94.83%,91.38% and 93.10% respectively;the sensitivity of PCR-SSP method and FCM method was signiifcantly higher than that of the MLCT method,the difference being statistically signiifcant （P〈 0.05）;the 3 methods all had a higher speciifcity,there being no signiifcant difference （P〉 0.05）;PCR-SSP method had higher sensitivity and speciifcity.The positive predictive value were 94.92%,91.53%,92.31%,the neg-ative predictive value was 96.49%,92.98%,84.38% respectively.The positive predictive values of the 3 methods were high,and there was no signiifcant difference （P〉 0.05）;the negative predictive values of PCR-SSP method and FCM method were signiifcantly higher than that of the MLCT method,the difference being statistically sig-niifcant （P〈 0.05）;PCR-SSP method had higher positive predictive value and negative predictive value.Conclusion：The 3 kinds of detection method had a higher speciifcity and positive predictive value in the diagnosis of AS.The PCR-SSP method had higher sensitivi-ty,speciifcity,positive predictive value and negative predictive value.

关 键 词：脊柱炎 强直性 序列特异性引物-聚合酶链式反应 流式细胞仪 微量淋巴细胞毒 HLA-B27 比较分析 

分 类 号：R593.23[医药卫生—内科学]