微波预处理对水稻秸秆糖化率与成分和结构的影响  

Effects of Microwave Pretreatment on Enzymatic Saccharification and Lignocellulosic Structure of Rice Straw

作　　者：马欢[1] 刘伟伟[2,3] 刘萍[4] 程备久[1] 朱苏文[1] 吴跃进[5] 

机构地区：[1]安徽农业大学生命科学学院,合肥230036 [2]安徽农业大学工学院,合肥230036 [3]中国科学院等离子体物理研究所,合肥230031 [4]莱斯大学生物工程学院,休斯敦77030 [5]中国科学院合肥物质科学研究院技术生物与农业工程研究所,合肥230031

出　　处：《农业机械学报》2014年第10期180-186,共7页Transactions of the Chinese Society for Agricultural Machinery

基　　金：安徽省科技计划资助项目(11010402100);安徽省高校省级自然科学研究资助项目(KJ2014A073)

摘　　要：利用微波辐照对水稻秸秆进行预处理,研究了优化条件下以水为媒介的微波辐照对秸秆酶解糖化率及酶吸附能力的影响。通过预处理前后秸秆主要化学成分的变化,以及FTIR、XRD和AFM对其表面形貌结构的表征,研究了微波促进水稻秸秆酶解的机制。结果表明:微波辐照可以破坏水稻秸秆表面特殊的'角质-双硅层'结构和木质素-半纤维素复合体,去除部分硅和木质素含量,因此能有效减少秸秆对酶的吸附,同时提高纤维素酶的酶解效率。Microwave irradiation was employed as pretreatment for rice straw.The effects of microwave on enzymatic saccarification and lignocellulosic structure of rice straw,as well as its adsorption ability of enzyme,were investigated under the optimal conditions of pretreatment.The chemical composition analysis and structural properties determination by Fourier transform infrared spectroscopy and atomic force microscopy further confirmed that microwave pretreatment could disrupt the silicified waxy surface,break down lignin-hemicellulose complex and partially remove silicon and lignin,which could be favorable for the enhancement of enzymatic saccharification of rice straw and reduction of its adsorption of enzymes.However,X-ray diffraction analysis revealed that microwave irradiation had no significant influence on crystalline structure of cellulose.

关 键 词：水稻秸秆 微波 预处理 糖化率 表征 

分 类 号：S216[农业科学—农业机械化工程] X712[农业科学—农业工程]