益气清瘟解毒合剂所含益气法中药抗流感免疫炎性损伤的机制  

Mechanism of tonifying Qi traditional Chinese medicines contained in Yiqi Qingwen Jiedu mixture against influenza immune inflammatory injury

作　　者：徐红日[1] 王成祥[1] 王兰[1] 周平安[2] 殷人易[3] 姜良铎[1] 王惠芳[4] 

机构地区：[1]北京中医药大学东直门医院,北京100700 [2]北京中医药大学东方医院,北京100078 [3]武汉市第一医院,湖北武汉430022 [4]解放军防化研究院,北京102205

出　　处：《中国中药杂志》2014年第20期4020-4026,共7页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(30873254)

摘　　要：目的： 通过动态观察益气清瘟解毒合剂中所含益气法中药对流感病毒感染小鼠肺中炎性细胞因子mRNA的表达及肺组织病理损伤的影响，探讨益气法中药抗流感病毒感染所致小鼠肺组织免疫炎性损伤的机制。 方法： 在流感病毒亚洲甲型鼠肺适应株FM1感染小鼠后的不同时相，采用反转录-聚合酶链式反应（RT-PCR）法动态观察益气清瘟解毒合剂中所含益气法中药对小鼠肺中TNF-α，IL-1，IL-6，IL-10，IFN-γ等5种炎性细胞因子mRNA表达的影响，并观察经益气法中药干预后小鼠病毒性肺炎肺组织病理损伤的改变。 结果： ①益气法中药在FM1感染后第1～5天显著降低小鼠肺中TNF-α mRNA的表达，在感染后第7天明显降低小鼠肺中IL-1 mRNA的表达，在感染后第3天可能升高小鼠肺中IL-1 mRNA的表达，在感染后第3～7天明显降低小鼠肺中IL-6 mRNA的表达，在感染后第3～7天显著升高IL-10 mRNA的表达，在感染后第1天显著升高IFN-γ mRNA的表达。②益气法中药能够显著抑制并修复FM1感染所致小鼠肺组织免疫炎性损伤，其作用在流感病毒FM1感染后第3～7天最为突出。 结论： 益气清瘟解毒合剂中所含益气法中药可能通过调节流感病毒感染机体失控的炎性细胞因子的基因表达，对抗肺组织的流感免疫炎性损伤，并促进炎性损伤的修复。Objective： To observe the impact of tonifying Qi traditional Chinese medicines contained in Yiqi Qingwen Jiedu mixture on mRNA expression of lung inflammatory cytokines and pulmonary pathological injury of mice infected by influenza virus, in order to discuss the mechanism of tonifying Qi traditional Chinese medicines against pulmonary immune inflammatory injury of infected mice. Method： In different time phases after mice were infected with influenza virus FM1, the RT-PCR method was adopted to observe the impact of tonifying Qi traditional Chinese medicines contained in Yiqi Qingwen Jiedu mixture on five inflammatory cytokines TNF-α, IL-1, IL-6, IL-10 and IFN-γ, and the changes in pulmonary pathological injury of mice with viral pneumonia after intervention with tonifying qi traditional Chinese medicines. Result： ①Tonifying Qi traditional Chinese medicines significantly reduced the mRNA expression of TNF-α at 1-5 d and IL-1 mRNA expression at 7 d, may increase IL-1 mRNA expression in mouse lung at 3 d, significantly reduced IL-6 mRNA expression in mouse lung and increased IL-10 mRNA expression at 3-7 d, and significantly increased IFN-γ mRNA expression at 1 d. ②Tonifying Qi traditional Chinese medicines could significantly inhibited and repaired pulmonary immune inflammatory injury of mice infected by FM1, which was most remarkable at 3-7 d after the infection with influenza virus FM1. Conclusion： Tonifying Qi traditional Chinese medicines contained in Yiqi Qingwen Jiedu mixture could resist pulmonary immune inflammatory injury and repair inflammatory injury by regulating the mRNA expression of imbalance inflammatory cytokines of organisms infected with influenza virus.

关 键 词：益气清瘟解毒合剂 益气法 流感 炎性损伤 机制 

分 类 号：R285.5[医药卫生—中药学]