分光光度法测定柿蒂中总三萜酸的含量  

Determination of the Content of Triterpenoidic Acid in Kaki Calyx

作　　者：任蕾[1] 冯海燕[1] 杨晓辉[1] 雷霓[1] 

机构地区：[1]石家庄学院化工学院,河北石家庄050035

出　　处：《石家庄学院学报》2014年第6期28-30,共3页Journal of Shijiazhuang University

基　　金：石家庄学院自然科学研究启动基金(12YB011)

摘　　要：测定中药柿蒂中总三萜酸的含量,以药材中指标性成分熊果酸为对照品,采用5%香草醛-冰醋酸、高氯酸显色后,分光光度法测定柿蒂提取液中总三萜酸的含量.结果显示:三萜酸含量在2.32-16.24μg/mL范围内呈良好线性关系,其回归方程为:A=0.056 7C+0.000 286,r=0.998 5.柿蒂中总三萜酸含量为5.93%,加样回收率平均为103.09%.该法操作简便,重现性好,结果可靠,可用于柿蒂药材中三萜酸成分的质量控制.The content of triterpenoidic acid in Kaki calyx is determined. With ursolic acid as the reference,5%vanillin-acetic acid and perchloric acid as the developer,the spectrophotometric method is used for determining the triterpenoidic acid in Kaki calyx. Results show that the calibration curve is linear（r=0.9985） in the range of 2.32~16.24 μg/mL,and that the regression equation is A=0.0567C＋0.000286. The content of triterpenoidic acid in Kaki calyx is about 5. 93% and the average recovery is 103.09%. Therefore,the method is simple,accurate and reproducible,and it could be used for monitoring the quality of triterpenoidic acid in Kaki calyx.

关 键 词：柿蒂 三萜酸 含量测定 分光光度法 

分 类 号：R284.1[医药卫生—中药学]