高速离心分配色谱法去除夏天无总生物碱中荷包牡丹碱的工艺研究  

Study on removing(-)-bicuculline from Corydalis Decumbentis Rhizoma total alkaloids by fast centrifugal partition chromatography

作　　者：谢欣辛[1] 孙百玲[1] 杨义芳[1] 

机构地区：[1]中国医药工业研究总院上海医药工业研究院,创新药物与制药工艺国家重点实验室,上海200040

出　　处：《中草药》2014年第19期2787-2792,共6页Chinese Traditional and Herbal Drugs

基　　金：国家"重大新药创制"科技重大专项(2009ZX09301-007);2012年上海市国际合作项目(12120701400);上海市自然科学基金资助项目(09ZR1431100);2010年上海市中药现代化专项资助项目(10DZ1971300);2010年上海市科委西部开发科技合作项目(10495801000)

摘　　要：目的采用高速离心分配色谱(FCPC)方法去除夏天无总生物碱中的毒性成分荷包牡丹碱(BI)。方法以体积流量、转速、样品浓度为考察因素,通过正交试验优化各工艺参数,同时对优化后的工艺结果与溶剂萃取法比较,并对最优工艺进行线性放大实验。结果最佳工艺为样品质量浓度80 mg/mL,转速1 200 r/min,体积流量4 mL/min。通过放大实验制得相应去除BI的夏天无总生物碱部位,BI去除率>94.0%,其余生物碱回收率>85.0%,优于传统溶剂萃取法。该工艺分离时间短,重现性好,操作简便。结论该工艺操作简单、快速、环保,不仅为提高夏天无总生物碱的安全性提供思路,而且为去除毒性成分的夏天无总生物碱产业化道路提供了方法。Objective Using fast centrifugal partition chromatography （FCPC） to remove （-）-bicuculline from Corydalis DecumbentisRhizomatotalalkaloids. Metbods The technological parameters of orthogonal experiment of FCPC was optimized by flow rate, rotation speed, and loading sample size. The optimized process was compared with the solvent extraction method, and the linear amplification experiments were carried out using the optimal process. Results The optimized parameters were as follows： rotation speed was 1 200 r/min, flow rate was 4 mL/min, and loading sample size was 80 mg/mL. The new prepared alkaloids fraction of Corydalis Decumbentis Rhizoma using amplification experiments has 〉 94.0% removed rate of BI and 〉85.0% recovery of the other alkaloids, which were higher than those of the solvent extraction method. This simple method with good repeatability could be completed in a short time. Conclusion The simple and fast method could improve the safety of Corydalis Decumbens Rhizoma total alkaloids, and provide the industrialization of non-toxic total alkaloids.

关 键 词：高速离心分配色谱 夏天无 总生物碱 去除率 荷包牡丹碱 正交试验 

分 类 号：R284.2[医药卫生—中药学]