吴茱萸汤外翻肠囊吸收成分与原药中各成分相关关系研究  

Correlation between absorption ingredients in Wuzhuyu Decoction and uptake amount in everted intestinal sac

作　　者：许永崧 潘学强[1] 龚慕辛[1] 贺蕊[1] 卢旭然[1] 尚雅文 

机构地区：[1]首都医科大学中医药学院,北京100069

出　　处：《中草药》2014年第17期2490-2498,共9页Chinese Traditional and Herbal Drugs

基　　金：北京市教育委员会科技计划面上项目(SQKM201210025011);首都中医药研究专项重点课题(13ZY02)

摘　　要：目的研究吴茱萸汤中9种成分的量对其自身吸收进入外翻肠囊成分量的影响,以指导吴茱萸汤改善偏头痛模型鼠指标的样品的调配。方法采用大鼠外翻肠囊模型获取10种吴茱萸汤吸收样品,以HPLC-DAD对吴茱萸汤及外翻肠囊吸收样品中9种成分进行定量、半定量,得到各成分在吴茱萸汤中的量以及在肠囊中的累积吸收量。用偏最小二乘回归法(partial least squares regression,PLSR)对吴茱萸汤中各成分的量及总体吸收量进行相关分析。结果在实验质量浓度范围内,柠檬黄素-3-O-β-D-葡萄糖苷、人参皂苷Rg1、6-姜辣素、吴茱萸苦素在吴茱萸汤中质量浓度越高,吸收入肠囊的总量越多;而人参皂苷Re、人参皂苷Rb1、柠檬苦素、吴茱萸碱、吴茱萸次碱则相反。成分之间对吸收的相互影响关系十分复杂。结论在根据吸收谱-效相关分析结果调配吴茱萸汤样品时,应考虑成分吸收的相互影响关系以达到较佳疗效。Objective To explore the influence of the contents of nine ingredients in Wuzhuyu decoction on its own uptake amount into the everted intestinal sac, in order to blend Wuzhuyu decoction samples to improve the index of migraine model rats. Methods The everted intestinal sac model was established to determine the absorption samples of 10 kinds of Wuzhuyu decoction. HPLC-DAD method was used to analyze the contents of nine ingredients in Wuzhuyu decoction and absorption samples quantitatively and semi-quantitatively. The partial least squares regression method was used to correlate the contents in the decoction and the total absorption quantity of nine ingredients. Results In the experimental concentration range, the higher the concentration of limocitrin-3-O-β-D-glucoside, ginsenoside Rg1, 6-gingerol, and ruteavin in the decoction were, the more they were absorbed into the intestine sac, while ginsenosides Re, Rb1, limonin, evodiamine, and rutaecarpine were contrary. The interaction of different ingredients on each other＇s absorption was complicated. Conclusion The above relationship should be considered to obtain the satisfactory samples of Wuzhuyu decoction.

关 键 词：吴茱萸汤 外翻肠囊 吸收 偏最小二乘回归 相关分析 

分 类 号：R285.5[医药卫生—中药学]