珠子参种质资源遗传多样性的ISSR分析  

ISSR Genetic diversity analysis of Panax japonicus var. major by molecular makers

作　　者：王薇[1,2] 刘超[1] 崔九成[1,2] 宋小妹[1,2] 梅其炳 岳正刚 

机构地区：[1]陕西中医学院,陕西咸阳712046 [2]秦巴山区中药发展协同创新中心,陕西西安710032

出　　处：《中草药》2014年第17期2525-2529,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81102805/H2804);陕西省科技统筹创新工程项目(2011KTCQ03-02);陕西省教育厅项目(2010JK502);陕西省中医管理局专项科研计划项目(zy08)

摘　　要：目的研究珠子参Panax japonicus种质资源的遗传多样性。方法采用ISSR分子标记技术,通过聚类分析和主坐标分析,研究3个主要产地珠子参的19个样本的遗传多样性和亲缘关系。结果 13条ISSR引物扩增出181条带,其中有166条呈现多态性,占91.71%,遗传相似系数变化范围0.60~0.83。聚类分析和主坐标分析结果一致,将源于同一地区的珠子参样本聚在一起,呈现出一定的地域性分布规律。结论珠子参样本之间的遗传多样性水平较高,种质间的亲缘关系和地理位置有一定关系。Objective To analyze the genetic diversity of Panax japonicus var. major by inter simple sequence repeat（ISSR） molecular makers. Methods Genetic diversity of 19 samples from main production areas was investigated by ISSR and analyzed by principal coordinate analysis and UPGMA cluster analysis. Results A total of 181 bands were generated by 13 ISSR primers, among which 166 bands（91.71%） were polymorphic bands（PPB）. The coefficient of genetic similarity ranged from 0.60 to 0.83. The results of UPGMA cluster analysis and principal coordinate analysis showed that the genetic diversity of P. japonicus var. major from the same region presented a geographical distribution regularity. Conclusion The results of ISSR analysis reveals that P. japonicus var. major has a high genetic diversity level and the genetic relationship closely contacts with the geographical location.

关 键 词：珠子参 ISSR 聚类分析 主坐标分析 遗传多样性 

分 类 号：R282.12[医药卫生—中药学]