高良姜素分子印迹聚合物分子识别性研究  

Molecular recognition of galangin molecular imprinting polymer microsphere

作　　者：姚杰[1] 乔华[1] 吕俊杰[1] 孙体健[1] 

机构地区：[1]山西医科大学基础医学院,山西太原030001

出　　处：《中草药》2014年第18期2614-2618,共5页Chinese Traditional and Herbal Drugs

基　　金：山西医科大学科技创新基金资助项目(C01201005);山西医科大学青年基金资助项目(02201309)

摘　　要：目的探讨高良姜素分子印迹聚合物(GMIP)的特异性分子识别能力。方法以高良姜素为模板分子,分别采用四氢呋喃、乙腈、丙酮为致孔剂,以丙烯酰胺(AM)为功能单体,以乙二醇二甲基丙烯酸酯(EGDMA)为交联剂,采用热引发聚合的方法合成GMIP;采用扫描电子显微镜(SEM)、傅里叶红外光谱(FTIR)扫描对其进行性能表征;运用平衡结合试验研究聚合物的吸附特性和选择识别能力。结果 Scathard模型分析表明,该GMIP在识别高良姜素分子的过程中存在2类不同的结合位点;高亲和力结合位点的离解常数(Kd1)=0.961 mmol/L,最大表观结合常数(Qmax1)=19.79μmol/g;低亲和力结合位点的离解常数(Kd2)=0.101 mmol/L,最大表观结合常数(Qmax2)=51.09μmol/g。结论 GMIP对高良姜素分子具有特异的吸附和识别能力,为天然产物中高效分离纯化活性成分高良姜素提供了一种新型材料。Objective To investigate the specific molecular recognition ability for galangin molecular imprinting polymer （GMIPI Methods GMIP was prepared through the method of thermal polymerization with galangin as template molecular, acrylamide （AM as fimctional monomer, ethyleneglycol dimethacrylate （EGDMA） as cross-linker, and tetrahydrogtran, acetonitrile, and acetone as pot forming agents, respectively. The GMIP was characterized by infrared spectroscopy and scanning electron microscopy （SEM）. I addition, the GMIP was investigated in equilibrium binding experiment to evaluate its adsorption property and selective recognitior Results The Scathard model analysis showed that two kinds of binding sites existed in the GMIP. The dissociation constant （Kd） an apparent maximum binding constant （Qrr^x） were Kdl = 0.961 mmol/L, Qmaxl = 19.79 μmol/g for high affinity binding sites and Kd2 ： 0.101 mmol/L, Qmax2 = 51.09 μmol/g for low affinity binding sites, respectively. Conclusion GMIP has the specific adsorption an recognition capabilities to the galangin molecules. It can be a novel material for the separation and purification ofthe active ingredien! from natural products.

关 键 词：高良姜素 分子印迹聚合物 分子识别 离解常数 最大表观结合常数 分子印迹技术 

分 类 号：R283.6[医药卫生—中药学]