虾夷扇贝脓胞病病原查氏弧菌的PCR快速检测  

Rapid Detection of Pathogen Vibrio chagasii from Abscess Yesso Scallop by PCR Technique

作　　者：滕炜鸣[1] 李文姬[1] 张明[1] 李石磊[1] 刘项峰[1] 付成东[1] 刘忠颖[1] 

机构地区：[1]辽宁省海洋水产科学研究院,辽宁省应用海洋生物技术开放实验室,辽宁省水产分子生物学重点实验室,辽宁大连116023

出　　处：《水产科学》2014年第10期649-653,共5页Fisheries Science

基　　金：国家海洋公益性行业科研专项(200805037);辽宁省科技计划项目(2011203003);大连市科学技术基金资助项目(2011J21DW029)

摘　　要：应用PCR技术,建立一种能够快速有效检测出虾夷扇贝脓胞病致病病原查氏弧菌的方法。根据查氏弧菌的GyrB基因的高保守区序列,设计1对扩增片段为144bp的引物,并利用PCR技术对其进行了特异性和敏感性检测。试验结果表明,该方法对查氏弧菌DNA的检测呈特异性,每个PCR反应检测的敏感度为0.43pg的DNA和3.9cuf的细菌。由人工感染和海区取样的虾夷扇贝的病灶组织中可以检测出相应的病原菌,说明本方法有效可行。The PRC primer was designed according to the hypervariable region sequence of GyrB gene in Vibrio chagasii in GenBank and applied to amplify a 144 bp DNA fragment in order to develop an effective method for the identification of V.chagasiiin yesso scallop Patinopecten yessoensis diseased with abscess.The primer showed sensitivity of 0.43 pg with detection limit of 3.9×10^-3cfu,and the PCR amplification was specific for the extracted DNA fromV.chagasii,instead of V.vulnificus,V.alginolyticus,V.alginolyticus,and V.splendidus.The findings indicate that the PCR offers sensitive and specific means for the detection of V.chagasii,and the recognition of V.chagasii from diseased yesso scallop.

关 键 词：虾夷扇贝 脓胞病 查氏弧菌 PCR 快速检测 

分 类 号：S944.4[农业科学—水产养殖]