PRNCR1基因沉默对于胃癌细胞BGC-823增殖活性的影响  

Effect of PRNCR1 Gene Silencing on Proliferation Activity in BGC-823 Cells of Gastric Cancer

作　　者：黄重发 付启梅 周怿 朱清 

机构地区：[1]解放军454医院消化科,南京210002

出　　处：《解放军医药杂志》2014年第10期47-50,共4页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

摘　　要：目的比较胃癌及其癌旁组织中PRNCR1含量;设计并合成针对PRNCR1基因的siRNA,转染人胃癌细胞BGC-823,观察PRNCR1含量改变对BGC-823细胞增殖活性的影响。方法 Real Time-PCR检测10对胃癌及其癌旁组织中PRNCR1含量;化学法合成针对PRNCR1的siRNAs,脂质体法转染至BGC-823细胞,转染分为细胞对照组、转染对照组、错义序列组和siRNA转染组。转染后48 h收集细胞,Real Time-PCR检测转染后细胞内PRNCR1含量变化;CCK-8检测肿瘤细胞对数生长期基因干预对于增殖活性的影响。结果 PRNCR1在胃癌组织中含量明显高于癌旁组织(P<0.05)。与细胞对照组比较,siRNA转染组细胞中PRNCR1含量明显降低,细胞增殖活性明显受到抑制(P<0.01);而转染对照组、错义序列组与细胞对照组比较,PRNCR1含量、细胞增殖活性均无明显差异(P>0.05)。结论 PRNCR1沉默可显著抑制BGC-823细胞增殖活性。Objective To compare the PRNCR1 content in gastric cancer tissues and adjacent tissues, siRNAs for PRNCR1 gene was designed and composed to transfect the human BGC-823 cells, and the effect of PRNCR1 content change on proliferation activity in BGC-823 cells was also observed. Methods PRNCR1 contents in 10 pairs of gastric cancer tissues and adjacent tissues were collected by Real Time-PCR （ RT-PCR） method; chemically synthetic siRNAs for PRNCR1 were transfected into BGC-823 cells by liposomes method, and then were divided into cell control group, transfection control group, noncoding group and siRNA transfection group. Cells were collected 48 h after transfection, and PRNCR1 content change was detected by RT-PCR method;CCK-8 method was used to detect the effect of genetic in-tervention on proliferative activity of tumor cells during the logarithmic growth phase. Results PRNCR1 content in the tumor tissues was significantly higher than that in the adjacent tissues （ P〈0. 05 ）; compared with those in cell control group, PRNCR1 level in siRNA transfection group was significantly reduced ,and proliferation activity of cells was signifi-cantly inhibited （P〈0. 01）;compared with those in cell control group, there were no significant differences in the PRN-CR1 content and proliferation activity of cells in transfection control group or noncoding group （P〉0. 05）. Conclusion PRNCR1 gene silencing can effectively inhibit proliferation activity of BGC-823 cells.

关 键 词：PRNCR1 胃肿瘤 RNA 小分子干扰 细胞增殖 

分 类 号：R342.2[医药卫生—基础医学] R735.2