乙肝病毒DNA定量水平和乙型肝炎病毒标志物定量检测指标相关性研究  

Correlation Research on Quantitative Level of Hepatitis B Virus DNA and Quantitative Detection Index Associated to Hepatitis B Virus Markers

作　　者：杨勇 王占科 陈鑫 钟莹 

机构地区：[1]解放军94医院感染性疾病科,南昌330002 [2]解放军94医院检验科,南昌330002

出　　处：《解放军医药杂志》2014年第10期78-81,共4页Medical & Pharmaceutical Journal of Chinese People’s Liberation Army

基　　金：国家科技支撑项目(2008BAI52B03)

摘　　要：目的探讨乙型肝炎病毒(HBV)标志物定量检测指标与HBV DNA定量水平的相关性。方法从2013年1月—2014年6月检验科采用酶联免疫吸附试验(ELISA)确诊的在感染性疾病科门诊就诊和住院的大三阳368例和小三阳507例中,各随机选取50例作为大三阳组和小三阳组。大三阳组和小三阳组的血标本同时做HBV标志物定量和HBV DNA定量检测。结果大三阳组HBsAg含量和HBV DNA定量检测结果均明显高于小三阳组(P<0.01);大三阳组HBV DNA定量阳性率明显高于小三阳组(P<0.01);HBV DNA阳性的大三阳患者HBV DNA含量与HBsAg、HBeAg含量均具有相关性(P<0.01)。结论 HBV DNA水平与HBV标志物中的HBsAg和HbeAg水平呈明显正相关;定量检测HBV DNA、HBsAg和HbeAg对判断HBV感染者传染性具有重要临床意义。Objective To investigate the correlation between quantitative detection index associated to Hepatitis b virus （ HBV） markers and the quantitative level of HBV DNA. Methods A total of 368 patients with great three posi-tive results and 507 patients with small three positive results were confirmed by using enzyme-linked immunosorbent assay （ ELISA） during January 2013 and June 2014 in infectious diseases department, and 50 patients were randomly selected from the 368 patients as great three positive results group （ group A） , and 50 patients were randomly selected from 507 patients as small three positive results group （ group B） . Blood samples of the two groups underwent quantitative detec-tions of HBV markers and HBV DNA simultaneously. Results HBsAg content and HBV DNA quantitative test results were significantly higher than those in group B （P〈0. 01）;positive rate of HBV DNA quantitation in group A was signif-icantly higher than that in group B （P〈0. 01）;HBV DNA content had correlation with the HBsAg and HBeAg contents in great three positive result patients with positive HBV DNA （P〈0. 01）. Conclusion HBV DNA level has obviously positive correlation with HBsAg and HbeAg levels, and quantitative detections of HBV DNA, HBsAg and HbeAg have important clinical significance for judging HBV infectivity.

关 键 词：乙型肝炎抗体 乙型肝炎抗原 乙型肝炎病毒 

分 类 号：R392.11[医药卫生—免疫学]