高产赖氨酸枯草芽孢杆菌的选育  

Selective breeding of Bacillus subtilis producing high-yield lysine

作　　者：欧荣娣[1,2] 邢月腾[1,2] 范觉鑫[1,2] 王来强[1] 李丽立[2] 张彬[1] 

机构地区：[1]湖南农业大学动物科学技术学院,湖南长沙410128 [2]中国科学院亚热带农业生态研究所动物生态营养与健康养殖联合实验室,农业生态工程重点实验室,湖南长沙410125

出　　处：《中国饲料》2014年第20期29-31,共3页China Feed

基　　金：国家自然科学基金青年基金项目(31302003);湖南省科技计划重点项目(2100CK2006);长沙市科技计划重点项目(K1201108-21)

摘　　要：为提高枯草芽孢杆菌产赖氨酸的能力,本试验拟从10株猪源枯草芽孢杆菌中,初步筛选出一株赖氨酸高产菌株。再以此菌株为出发菌株,采用紫外诱变的方法复筛出高产赖氨酸的突变株。结果发现,初筛得到的菌株YB83,其培养上清液中赖氨酸含量为179.39 mg/L,较LB液体培养基高出22.58%;经过紫外诱变后,得到一株遗传性稳定的突变菌株YB83-U5,其培养上清液中赖氨酸含量为277.49 mg/L,比原始菌株YB83提高了52.97%。To improve the ability of Bacillus subtilis in producing lysine,ten porcine Bacillus subtilis were applied to select and breed high-yield lysine strain.The strain as initial bacterium,which had highest lysine yield among them was treated with UV.The results showed that lysine content of supernatant of strain YB83 screened primarily was 179.39 mg/L, which was 22.58%higher than that of LB broth.After UV mutagenesis,the mutant strain YB83-U5 was steady hereditary, the lysine content of supernatant of which was 277.47 mg/mL,52.97%more than that of the original strain YB83.

关 键 词：枯草芽孢杆菌 赖氨酸 紫外诱变 

分 类 号：S816.79[农业科学—饲料科学]