苯酚对红鳍东方鲀肝胰脏CuZn-SOD和Mn-SOD基因表达的半定量分析  

Expression Analysis of CuZn-SOD and Mn-SOD Genes in Hepatopancreas of Takifugu rubripes after Phenol Exposure

作　　者：石洪玥[1] 崔培[1] 姜志强[2] 陈成勋[1] 

机构地区：[1]天津农学院水产学院天津市水产生态及养殖重点实验室,天津300384 [2]大连海洋大学农业部北方海水增养殖重点实验室,辽宁大连116023

出　　处：《天津农学院学报》2014年第3期1-6,共6页Journal of Tianjin Agricultural University

基　　金：辽宁省高等学校优秀人才支持计划项目"红鳍东方鲀遗传改良技术研究"(LR201010);国家海洋局公益性行业科研专项"海水鱼低碳高效养殖关键技术集成与示范"(2012418025);天津市科技支撑计划重点项目"河鲀鱼良繁体系构建及大规模繁育"(12ZCDZNC05900)

摘　　要：应用半定量RT-PCR法,研究苯酚对红鳍东方鲀(Takifugu rubripes)幼鱼肝胰脏CuZn-SOD和Mn-SOD基因相对表达量的影响。试验鱼饲养在苯酚浓度为0、0.18、0.32、0.56、1.00和1.35 mg/L的海水中,分别在苯酚暴露前和暴露4、8、12和16 d后取样。结果表明,在苯酚暴露前,鱼体肝胰脏中CuZn-SOD和Mn-SOD基因均有基础表达。在整个暴露时间(16 d)下,CuZn-SOD和Mn-SOD mRNA相对表达量呈现出逐渐上调的趋势,即苯酚诱导了两种基因的表达。在苯酚暴露第4天时,各浓度处理组CuZn-SOD mRNA和Mn-SOD mRNA的相对表达量均达峰值,显著高于暴露前水平(P<0.05)。The semi-quantitative RT-PCR method was applied to test the expression of CuZn-SOD gene and Mn-SOD gene in Takifugu rubripes hepatopancreas after phenol exposure. Fish was cultured in seawater containing 0, 0.18, 0.32, 0.56, 1.00 and 1.35 mg/L phenol, respectively, and was sampled before phenol exposure and after 4, 8, 12 and 16 d phenol exposure. The results show that basal expression of CuZn-SOD gene and Mn-SOD gene were detected in fish before phenol exposure. The relative expression levels of CuZn-SOD and Mn-SOD mRNA showed a trend of gradual up-regulation throughout the 16 days phenol exposure and the phenol induced the expression of CuZn-SOD and Mn-SOD gene. The mRNA expression level in each phenol exposure group peaked at the fourth day and was significantly higher than that of the phenol pre-exposure level （P〈0.05） .

关 键 词：苯酚 红鳍东方鲀 毒性实验 CUZN-SOD MN-SOD 

分 类 号：S941.91[农业科学—水产养殖]