HPLC-ICP-MS联用技术分析烟草中砷的形态  

Speciation analysis of arsenic in tobacco by HPLC-ICP-MS

作　　者：张春涛[1] 陆怡峰 李海霞[3] 李登科[1] 马立超[1] 孟庆乃[1] 姚鹤鸣[2] 叶鸿宇[1] 

机构地区：[1]上海烟草集团有限责任公司技术中心天津工作站,天津300163 [2]上海烟草集团有限责任公司技术中心,上海200082 [3]南开大学化学学院,天津300071

出　　处：《分析试验室》2014年第10期1233-1236,共4页Chinese Journal of Analysis Laboratory

摘　　要：采用高效液相色谱-电感耦合等离子体质谱（HPLC-ICP-MS）联用技术对烟草中砷的形态进行分析研究。烟草样品经超纯水提取,采用阴离子交换柱,以30 mmol/L碳酸铵水溶液作流动相,成功分离了亚砷酸（As（Ⅲ））、砷酸（As（V））、一甲基砷（MMA）和二甲基砷（DMA）,检出限依次为0.15,0.16,0.17,0.16 ng/mL,加标回收率在73.8%~107.6%之间,相对标准偏差（RSD）均小于8.0%（n=3）。A high performance liquid chromatography and inductively coupled plasma mass spectrometric （HPLC-ICP-MS） method has been developed for the determination of 4 arsenic species as arsenite （As（III） ）, arsenate （ As （ V ） ） monomethylarsonic acid （ MMA ） and dimethylaronic acid （ DMA ） in tobacco. Tobacco samples were pretreated by ultrapure water, and the separation was performed on an anion-exchange column with the mobile phase composition of 30 mmoL/L ammonium carbonate solution. The limits of detection （LOD） were 0.15 ng/mL （As（III））, 0.16 ng/mL （As（V））, 0.17 ng/mL （MMA） and 0.16 ng/mL （DMA）, respectively. The average spiked recoveries were between 73.8% and 107.6%, and relative standard deviations （ n = 3 ） were below 8.0%. This method has the advantages of simple operation, good separation effect, high sensitivity and fast analytical speed etc.

关 键 词：HPLC-ICP-MS 砷 形态分析 烟草 

分 类 号：O657.63[理学—分析化学]