激动β_3肾上腺素受体对载脂蛋白E基因敲除小鼠胰腺血管紧张素受体表达的影响  

Effect of β_3 adrenoceptor activation on expression of pancreatic angiotensin receptor in apoE/mice

作　　者：宋俊迎 李艳芳[1,2] 蒋志丽[1,2] 郭彦青[1,2] 

机构地区：[1]首都医科大学附属北京安贞医院急诊35病房 [2]北京市心肺血管疾病研究所,北京100029

出　　处：《中华老年心脑血管病杂志》2014年第10期1069-1072,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：国家自然科学基金(81270380)

摘　　要：目的探讨激动β3肾上腺素受体(β3-AR)对载脂蛋白E基因敲除(apoE-/-)小鼠血糖、胰岛素和胰腺血管紧张素Ⅱ受体(ATR)表达水平的影响。方法选用10周龄C57BL/6J小鼠10只为对照组(A组),另选10周龄apoE-/-小鼠50只,高脂饮食至36周龄时随机分为高脂模型组(B组)、阿托伐他汀阳性药物对照组(C组)、β3-AR激动剂小剂量组(D组)、β3-AR激动剂大剂量组(E组)和β3-AR拮抗剂组(F组),干预12周。48周时检测小鼠血糖和胰岛素水平;采用实时定量PCR和Western blot检测各组小鼠AT1R和AT2R表达水平。结果与A组比较,B组血糖、胰岛素明显升高,AT1R明显上调,AT2R明显下调(P<0.01);与B组比较,D组、E组血糖、胰岛素明显降低,AT1R明显下调,AT2R明显上调(P<0.01)。结论长期应用β3-AR激动剂通过下调apoE-/-老年高脂小鼠胰腺AT1R表达和上调AT2R表达,β3-AR与AT1R和AT2R存在交互作用,且与改善糖代谢紊乱有关。Objective To study the effect ofβ3 adrenoceptor （β3-AR）activation on serum blood glu-cose and insulin level and expression of pancreatic angiotensin receptor （ATR） in apoE/mice . Methods Ten wild-type C57BL/6J mice at the age of 10 weeks served as a normal control group （group A）and 50 apoE/mice at the age of 10 weeks fed with high fat diet to 36 weeks old were randomly divided into hyperlipidaemia model group （group B ） ,atorvastatin treatment group （group C） ,low β3-AR agonist dose group （group D） ,high β3-AR agonist dose group （group E） andβ3-AR antagonist group （group F） ,10 in each group .The mice were treated withβ3-AR ago-nist and antagonist for 12 weeks .Serum blood glucose and insulin levels in 48 weeks old mice were measured using an automatic biochemical detector .Expression of AT1 R and AT2 R in pancre-atic tissues of mice was detected by RT-PCR and Western blot ,respectively .Results The serum blood glucose and insulin levels were significantly higher ,the expression level of AT1 R was sig-nificantly higher while that of AT2R was significantly lower in group B than in group A （P〈0.01） .The serum blood glucose and insulin levels were significantly lower ,the expression level of AT1 R was significantly lower while that of AT2 R was significantly higher in groups D and E than in group B （ P〈0 .01） .Conclusion Long-term use of β3-AR agonist can improve blood glucose metabolism in apoE/mice by down-regulating the AT1 R expression and up-regulating the AT2 R expression ,indicating that β3-AR interacts with AT1 R and AT2 R .

关 键 词：载脂蛋白E类 小鼠  基因敲除 血糖 胰岛素 受体  血管紧张素 肾上腺素能Β激动剂 

分 类 号：R589.2[医药卫生—内分泌]