山东地区153例汉族丙型肝炎患者HCV基因分型研究  

Investigation of HCV genotypes in 153 patients with chronic hepatitis C in Shandong Han nationality

作　　者：刘义庆[1] 田文君[1] 渠滕 刘春梅[1] 许丽[2] 王盛华[1] 朱之炜 张炳昌[1] 

机构地区：[1]山东大学附属省立医院检验科,济南250021 [2]山东大学第二医院肝病科,济南250033 [3]山东省济南市体外诊断试剂工程技术研究中心,250101

出　　处：《检验医学与临床》2014年第19期2649-2650,共2页Laboratory Medicine and Clinic

基　　金：国家自然科学基金资助项目(81102220);山东省科学技术发展计划资助项目(2010G0020225);山东省自然基金资助项目(ZR2011HM019);山东省临床重点专科建设项目经费资助

摘　　要：目的了解近年来山东地区汉族丙型肝炎病毒(HCV)感染者基因型的分布特点及与HCV-RNA病毒载量的相关性。方法抽取山东省立医院门诊及住院汉族丙型肝炎患者实时荧光定量聚合酶链反应检测RNA阳性标本153例,用焦磷酸测序法进行基因分型。结果共检出5种基因亚型,1b、2a、1a、3a、3b比例依次为65.36%、28.76%、3.92%、1.32%、0.65%。1b亚型病毒载量高于2a,其他亚型病毒载量之间比较,差异无统计学意义(P>0.05)。结论山东地区汉族HCV基因型以1b为主,其次为2a,且1b亚型病毒载量高于2a。Objective To study the latest distribution of hepatitis C genotypes and the correlation between hepatitis C genotypes and viral loads among chronic hepatitis C patients of Han nationality in Shandong region .Meth-ods One hundred and fifty three HCV RNA positive cases from inpatients and outpatients of Han nationality in Shandong provincial hospital were detected by real-time fluorescent quantitative PCR （FQ-PCR） ,followed by pyro-phosphate sequencing method for genotyping .Results Five subtypes including HCV 1b ,2a ,1a ,3a ,3b were detected at frequencies of 65 .36% ,28 .76% ,3 .92% ,1 .31% ,0 .65% .HCV1b was associated with a significantly higher viral load compared to HCV2a（P〈 0 .05） .There was no significant differences among the other subtypes .Conclusion HCV 1b is the most predominant subtypes among hepatitis C patients of Han nationality in Shandong ,followed by 2a ,and the viral load of HCV 1b was higher than 2a .

关 键 词：丙型肝炎病毒 基因型 焦磷酸测序 

分 类 号：R373.2[医药卫生—病原生物学]