不同外源激素对绞股蓝愈伤组织诱导的影响  

Effects of Different and Concentration of Exogenous Hormone on Callus Induction of Gynostemma Pentaphyllum

作　　者：蔡正旺[1] 刘静[1] 

机构地区：[1]安康学院农学与生命科学学院,陕西安康725000

出　　处：《安康学院学报》2014年第5期108-111,共4页Journal of Ankang University

基　　金：安康学院高层次人才科研启动项目(AYQDZR201212)

摘　　要：以绞股蓝茎尖、茎段、叶片为外植体,研究了不同种类、不同浓度配比的外源激素对绞股蓝愈伤组织诱导的影响。结果表明:茎尖在培养基MS+6-BA 1.0 mg·L-1+NAA 0.02 mg·L-1中的诱导效果最好,其诱导率为75.0%;茎段在培养基MS+6-BA 2.0 mg·L-1+NAA 0.2 mg·L-1中的诱导效果最好,其诱导率为81.8%;叶片在培养基MS+6-BA 1.0 mg·L-1+NAA 0.15 mg·L-1中的诱导效果较好,其诱导率为69.2%。Gynostemma shoot tips,stems,leaves as explants to MS medium,of different types,different proportions of exogenous hor-mones on callus induction of Gynostemma. The results show that：the concentration of exogenous hormones is different for different shoot tip,tem,and leaf callus induction. In the medium MS＋6- BA 1.0 mg/L＋NAA 0.02mg/L in the shoot tips of the best induction effect, the induction rate of 75.0%, and the callus growth is good;in the medium MS ＋6- BA2.0mg/L＋ NAA 0.2mg/L in stems induced the best,the induction rate was 81.8%,and the callus grew well;n the medium MS＋6- BA 1.0mg/L＋NAA 0.15mg/L leaves induced ef-fect is better,its induction rate of 69.2%.

关 键 词：外源激素 绞股蓝 愈伤组织 诱导 影响 

分 类 号：Q943.1[生物学—植物学]