儿童支气管哮喘患者外周血Th17百分数及FOXP3水平的变化及意义  

Changes and clinical significance of Th17 percentage and FOXP3 concentration in peripheral blood of children with bronchial asthma

作　　者：张晴菊[1] 龚芳[1] 申卫红[1] 

机构地区：[1]南通大学第三附属医院检验科,江苏无锡214041

出　　处：《国际检验医学杂志》2014年第18期2451-2452,共2页International Journal of Laboratory Medicine

基　　金：无锡市医院管理中心医学技术重大扶持项目(YGZF1101)

摘　　要：目的探讨外周血Th17细胞百分数和叉头状转录因子P3(FOXP3)水平在儿童支气管哮喘患者中的变化及意义。方法选取支气管哮喘患儿(哮喘组)和健康儿童(对照组)各30例,采用流式细胞术检测外周血Th17细胞及CD4+CD25+调节性T细胞的百分数、实时荧光定量PCR检测外周血FOXP3mRNA的表达,ELISA法检测血浆中IL-17和IL-10的表达水平。结果哮喘组外周血Th17细胞百分数及血浆IL-17水平均高于对照组(P<0.05);哮喘组外周血FOXP3表达及血浆IL-10水平均低于对照组(P<0.05)。结论 Th17/FOXP3的失衡可能在儿童支气管哮喘的发病机制中起重要作用。Objective To investigate the percentage of Th17 cells and FOXP3 concentration in peripheral blood of children with bronchial asthma and their clinical significance .Methods Thirty children with bronchial asthma and thirty healthy children as con-trol group were enrolled in the study .The percentage of Th17 and CD4＋CD25＋ Treg cells in the peripheral blood were determined by flow cytometry（FCM） .The mRNA expression of FOXP3 in the peripheral blood was determined by quantitative real-time PCR . The concentrations of IL-17 and IL-10 in plasma were measured by using ELISA .Results In children with bronchial asthma ,the proportions of Th17 cells in the peripheral blood and concentration of IL-17 in plasma increased ,while the expression of FOXP3 in the peripheral blood and concentration of IL-10 in plasma decreased .Conclusion The imbalance of Th17/FOXP3 may contribute to the proceeding of bronchial asthma in children .

关 键 词：支气管哮喘 辅助性T细胞 调节性T细胞 叉头状转录因子P3 

分 类 号：R725.6[医药卫生—儿科]