酶联免疫吸附试验试剂盒稳定剂的研制与应用  

The development and application of stabilizers for ELISA test kit

作　　者：武向丽[1] 程华[2] 史蕾[3] 崔硕[2] 闫静辉[2] 

机构地区：[1]河北省邯郸市中心医院药剂科,河北邯郸056001 [2]河北省科学院生物研究所,河北石家庄050081 [3]河北师范大学生命科学学院,河北石家庄050024

出　　处：《国际检验医学杂志》2014年第18期2527-2528,共2页International Journal of Laboratory Medicine

摘　　要：目的研制酶联免疫吸附试验试剂盒中各组分的稳定剂,使得试剂盒保存期达到国家相关规定。方法采用经验法和正交法相结合的办法,配制一系列稳定剂配方,对酶标板、酶标抗体及标准品等进行处理,通过对照试验,筛选出稳定效果最好的配方。结果筛选到一种包被稳定剂(质量分数:牛血清清蛋白0.5%,明胶0.25%,海藻糖5%,PEG4000 0.1%),一种酶标抗体稳定剂(质量分数:牛血清清蛋白1%,蛋白胨1%,蔗糖10%,海藻糖5%,PEG4000 0.25%)。结论在稳定剂作用下,酶联免疫吸附试验试剂盒可在4℃条件稳定放置1年。Objective To develop ELISA kit stabilizer for each component ,with which the shelf life of kit could meet the com-mercial need .Methods A series of stabilizers for microplate ,enzyme-labeled antibody and standards were prepared by the means of experience and orthogonal approach ,through controlled trials ,screening out the best stabilizer formulations .Results A kind of coa-ted stabilizer （mass fraction ：bovine serum albumin 0 .5% ,gelatin 0 .25% ,trehalose 5% ,PEG4000 0 .1% ） and a kind of enzyme la-beled antibody stabilizer （mass fraction ：Bovine serum albumin 1% ,peptone 1% ,sucrose 10% ,trehalose 5% ,PEG4000 0 .25% ） were screened out .Conclusion The ELISA kits can be stable at 4 ℃ for 1 year after treated with the obtained stabilizers .

关 键 词：酶联免疫试剂盒 酶标板 酶标抗体 稳定剂 

分 类 号：R446.6[医药卫生—诊断学]